A potential H-DNA element in the MUC1 promoter does not influence transcription.
Authors Pahwa G S; Maher L J 3rd; Hollingsworth M A;
JournalJ Biol Chem
PubMed ID8900124
A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, ... More
B-Raf is dispensable for K-Ras-mediated oncogenesis in human cancer cells.
AuthorsKim JS, Lee C, Foxworth A, Waldman T,
JournalCancer Res
PubMed ID15026326
Oncogenic mutations in B-Raf and Kirsten-Ras (K-Ras) are mutually exclusive during human cancer pathogenesis. In an effort to study the biological basis of this epistasis, gene targeting was used to create isogenic sets of human cancer cells differing only in presence or absence of endogenous oncogenic K-Ras or wild-type B-Raf. ... More