GTP hydrolysis by the Rho family GTPase TC10 promotes exocytic vesicle fusion.
AuthorsKawase K, Nakamura T, Takaya A, Aoki K, Namikawa K, Kiyama H, Inagaki S, Takemoto H, Saltiel AR, Matsuda M,
JournalDev Cell
PubMed ID16950130
'TC10, a Rho family GTPase, has been shown to play an important role in the exocytosis of GLUT4 and other proteins, primarily by tethering the vesicles at the plasma membrane. Using a newly developed probe based on fluorescence resonance energy transfer, we found that TC10 activity at tethered vesicles dropped ... More
Biological activity of follistatin isoforms and follistatin-like-3 is dependent on differential cell surface binding and specificity for activin, myostatin, and bone morphogenetic proteins.
AuthorsSidis Y, Mukherjee A, Keutmann H, Delbaere A, Sadatsuki M, Schneyer A,
JournalEndocrinology
PubMed ID16627583
Follistatin (FST) and FST-like-3 (FSTL3) are activin-binding and neutralization proteins that also bind myostatin. Three FST isoforms have been described that differ in tissue distribution and cell-surface binding activity, suggesting that the FST isoforms and FSTL3 may have some nonoverlapping biological actions. We produced recombinant FST isoforms and FSTL3 and ... More
A novel secreted endonuclease from Culex quinquefasciatus salivary glands.
AuthorsCalvo E, Ribeiro JM,
JournalJ Exp Biol
PubMed ID16809456
Previous analysis of the salivary gland transcriptome of Culex quinquefasciatus showed the potential presence of an endonuclease with sequence similarities to shrimp, crab and two tsetse salivary proteins. Indeed, not only was the cloned cDNA shown to encode an active double-stranded endonuclease, but also the same activity was demonstrated to ... More