Platinum™ SuperFi II DNA 聚合酶
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Platinum™ SuperFi II DNA 聚合酶
Invitrogen™

Platinum™ SuperFi II DNA 聚合酶

Invitrogen Platinum SuperFi II DNA 聚合酶是一款校读 DNA 聚合酶,将超高保真度与创新的缓冲液结合在一起,可较大限度提高 PCR 通用引物退火温度了解更多信息
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货号反应次数
123612505 x 500 反应
12361010100 次反应
12361050500 次反应
货号 12361250
价格(CNY)
38,711.00
Each
添加至购物车
反应次数:
5 x 500 反应
请求批量或定制报价
价格(CNY)
38,711.00
Each
添加至购物车
Invitrogen Platinum SuperFi II DNA 聚合酶是一款校读 DNA 聚合酶,将超高保真度与创新的缓冲液结合在一起,可较大限度提高 PCR 通用引物退火温度。它是克隆、突变和其它需要极佳序列准确度实验应用的理想选择。

Platinum SuperFi II DNA 聚合酶的特点包括:
•出色的 >300X Taq 保真度
• 通用引物退火温度为 60°C
• 具有出色的特异性、灵敏度和得率
• 可稳健地扩增难以扩增的靶标(例如纯度不佳的靶标、GC 含量 ˃65% 的靶标、需长片段 PCR 的靶标)

Platinum SuperFi II DNA 聚合酶是一种工程酶,具有高持续合成能力,并增强对 PCR 抑制剂的耐受性。它也可以实现快速循环方案和扩增长靶标(长达 20 kb)。Platinum 热启动技术使用专有抗体,可在 PCR 变性步骤之前抑制酶活性,防止非特异性扩增和引物降解。该技术还可在室温下进行反应构建,提高了灵敏度和得率。

由于 SuperFi II PCR 缓冲液的独特成分,按照一般设计规则设计的大多数引物对的退火温度为 60°C。缓冲液中的等稳定化分子可提高退火步骤中引物-模板的双重稳定性,并可增强特异性,而无需优化每个引物对的退火温度。使用 Platinum SuperFi II DNA 聚合酶,可以使用同一方案将不同的 PCR 测定共循环,该方案即使用通用引物退火温度和为待扩增的最长片段选择的延伸步骤。

Platinum SuperFi II DNA 多聚酶的应用:
•高保真 PCR
• 克隆和亚克隆
• 位点定向诱变
• 富含 GC 的模板的扩增
• 产生用于测序的模板
• 高通量 PCR
• 扩增纯度不佳的样品
• 长片段 PCR
• 快速 PCR

为提高便利性,我们提供 Platinum SuperFi II 预混液和 Platinum SuperFi II DNA 聚合酶,并提供即用型混合物和 SuperFi II PCR 缓冲液和 dNTP,因此减少了 PCR 反应构建过程中的移液步骤数量。也可以使用 Platinum SuperFi II Green PCR 预混液,此外,其还包含一种密度试剂和两种示踪染料,用于 PCR 产物直接上样到凝胶上,进一步从设置到最终分析简化了 PCR 的工作流程。

查看有关 Platinum SuperFi II DNA 聚合酶产品的更多信息›
规格
保真度(相对于 Taq)>300 X
热启动内置热启动
反应次数5 x 500 反应
突出端平末端
聚合酶Platinum SuperFi II DNA 聚合酶
数量5 x 500 次反应
反应形式独立
运输条件干冰
尺寸(最终产品)20 kb 或更小
适用于(应用)Hot-start PCR, High-fidelity PCR
高 GC PCR 扩增效果
反应速度快速
Unit SizeEach
内容与储存
五盒 Platinum SuperFi II Green PCR 预混液,每盒包含:
•0.5 mL Platinum SuperFi II DNA 聚合酶
• 6 x 1.25 mL SuperFi II 缓冲液 (5X)

足以进行2500次 50 μL 反应

常见问题解答 (FAQ)

What is the longest amplicon I can get using Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase enables amplification of long targets (up to 20 kb).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

I'm having a hard time amplifying/cloning low copy cDNAs via PCR. Do you have any suggestions for me?

You may want to consider using our Platinum SuperFi II DNA Polymerase (Cat. No. 12361010) and try optimizing your PCR conditions if needed. The Platinum SuperFi II DNA Polymerase demonstrates a superior detection sensitivity with as little as 0.4 ng of genomic DNA. It is also a good idea to include a positive control reaction in parallel to your test PCR to ensure there are no issues with your reagents during handling or use over time. Please note that control templates may be ordered through our GeneArt Gene Synthesis (de novo sequence with 100% guarantee delivered in a vector) or Strings (up to 3 Kb, blunt-ended dsDNA pools, cloned into ZeroBlunt TOPO vector, convenient for multiple controls) services.

What are your recommendations when working with long amplicons using Platinum SuperFi II DNA Polymerase?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.2 µM may also improve the results.

Can I use Platinum SuperFi DNA Polymerase cycling protocol and primer annealing temperature with Platinum SuperFi II DNA Polymerase?

Yes, Platinum SuperFi II DNA Polymerase works at both universal 60 degrees C annealing temperature and at annealing temperatures calculated with a Tm calculator.

Can I perform TA cloning with Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase produces blunt-ended PCR products that can be cloned directly into blunt-ended cloning vectors. TA cloning is also possible if 3' dA-overhangs are added after PCR. We recommend purifying the PCR products of Platinum SuperFi DNA Polymerase before adding the overhangs. The procedure for adding 3' dA-overhangs (TA cloning) includes the following steps:
- Purify the PCR product (e.g., with a PCR purification kit or phenol extraction/DNA precipitation). Before adding the overhangs, PCR product must be purified, as the strong proofreading activity of any remaining Platinum SuperFi DNA Polymerase will degrade the added 3' dA-overhangs.
- Perform 3' dA addition with a Taq DNA polymerase.
Reaction components:
Purified PCR product
0.2 mM dATP
1x Taq Buffer
1 U Taq DNA Polymerase
Incubate the reaction for 20 min at 72 degrees C.
- Proceed to TA cloning. For optimal ligation efficiency, we recommend using fresh PCR products, since 3' dA-overhangs can be lost during storage