Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2.
AuthorsStillemark-Billton P, Beck C, Borén J, Olofsson SO,
JournalJ Lipid Res
PubMed ID15520448
'In this study, we tested the hypothesis that two separate pathways, the two-step process and an apolipoprotein B (apoB) size-dependent lipidation process, give rise to different lipoproteins. Expression of apoB-100 and C-terminally truncated forms of apoB-100 in McA-RH7777 cells demonstrated that VLDL particles can be assembled by apoB size-dependent linear ... More
Cell stiffness and receptors: evidence for cytoskeletal subnetworks.
AuthorsHuang H, Sylvan J, Jonas M, Barresi R, So PT, Campbell KP, Lee RT,
JournalAm J Physiol Cell Physiol
PubMed ID15385268
'Viscoelastic models of cells often treat cells as homogeneous objects. However, studies have demonstrated that cellular properties are local and can change dramatically on the basis of the location probed. Because membrane receptors are linked in various ways to the intracellular space, with some receptors linking to the cytoskeleton and ... More
Isolation and characterization of microvessel endothelial cells from human mammary adipose tissue.
AuthorsHewett PW, Murray JC, Price EA, Watts ME, Woodcock M,
JournalIn Vitro Cell Dev Biol Anim
PubMed ID7686548
A method for the isolation and long-term culture of human microvessel endothelial cells from mammary adipose tissue (HuMMEC) obtained at breast reduction surgery has been developed. Pure cultures of HuMMEC were isolated by sequential digestion of the fat with collagenase and trypsin followed by specific selection of microvessel fragments with ... More
Human lung microvessel endothelial cells: isolation, culture, and characterization.
AuthorsHewett PW, Murray JC,
JournalMicrovasc Res
PubMed ID8412855
The pulmonary vasculature is of great physiological/pathological significance. We have isolated and cultured microvessel endothelial cells (HuLEC) from lung tissue obtained from lung transplant recipients by modification of published methods. Pure cultures of HuLEC were isolated by mechanical disaggregation of the tissue prior to sequential dispase and trypsin digestion to ... More
Expansion of functional endogenous antigen-specific CD4+CD25+ regulatory T cells from nonobese diabetic mice.
AuthorsMasteller EL, Warner MR, Tang Q, Tarbell KV, McDevitt H, Bluestone JA,
JournalJ Immunol
PubMed ID16116193
CD4+CD25+Foxp3+ regulatory T cells (T(reg)) are critical for controlling autoimmunity. Evidence suggests that T(reg) development, peripheral maintenance, and suppressive function are dependent on Ag specificity. However, there is little direct evidence that the T(reg) responsible for controlling autoimmunity in NOD mice or other natural settings are Ag specific. In fact, ... More
Binding of human endothelium to Ulex europaeus I-coated Dynabeads: application to the isolation of microvascular endothelium.
A major problem encountered when isolating human microvascular endothelium is the presence of contaminating cells such as fibroblasts that rapidly over-grow the endothelial cells. We describe here a simple, rapid technique for purifying endothelial cells derived from the microvasculature of neonatal foreskin and osteoarthritic and rheumatoid arthritic synovium. This technique ... More