HBSS, calcium, magnesium, no phenol red, 500 mL - FAQs

大多数培养基都含有酚红，会淬灭可见光波长范围的荧光染料。大多数培养基还含有自发荧光成分——如核黄素——会降低信噪比。我们提供的FluoroBrite DMEM和基于HEPES的活细胞成像溶液已针对荧光成像应用进行了优化。此外，我们还提供多种不含酚红的培养基。但是，如果这些产品都不是您的试验的合理选择，为此我们还提供BackDrop 背景抑制剂 ReadyProbes 试剂，将其加入可淬灭培养基的自发荧光。

一些类型的细胞会积聚酚红，阻碍使用荧光探针。酚红会淬灭可见光波长范围内的染料。此外，尽管酚红本身并不发荧光，但其中的各种杂质可能会发出荧光。我们有许多不含酚红的培养基可供选择。我们的活细胞成像溶液（基于HEPES）和FluoroBrite DMEM已经过优化，不含酚红且无自发荧光。

是的，请访问此页面(https://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf)浏览我们的无酚红培养基页面。

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.

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The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

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HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

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Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

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Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

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Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

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No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.

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No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

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Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.