Preparation of retinal explant cultures to study ex vivo tip endothelial cell responses.
AuthorsSawamiphak S, Ritter M, Acker-Palmer A,
JournalNat Protoc
PubMed ID20885378
This protocol details a culture technique for neonatal mouse retina that allows the assessment and quantification of acute responses of developing blood vessels to pharmacological manipulation. The technique has proven to be a useful tool for elucidating the molecular mechanisms that underlie the guidance of tip cells in the complex ... More
An evaluation of a four-channel multiplexed electrospray tandem mass spectrometry for higher throughput quantitative analysis.
Authors Morrison Denise; Davies Amy E; Watt Alan P;
JournalAnal Chem
PubMed ID11985324
A four-channel multiplexed electrospray inlet system (MUX) coupled to a triple quadrupole mass spectrometer was investigated as a higher throughput approach to quantitative analysis. Four discrete samples may be simultaneously analyzed by virtue of a rotating sampler with a concomitant 4-fold increase in analytical throughput. Although absolute sensitivity was reduced ... More