Method for Large-scale Production of hIPSC Spheroids.
AuthorsLemarié L,Courtial EJ,Sohier J
JournalBio-protocol
PubMed ID38618177
Stem cell spheroids are rapidly becoming essential tools for a diverse array of applications ranging from tissue engineering to 3D cell models and fundamental biology. Given the increasing prominence of biotechnology, there is a pressing need to develop more accessible, efficient, and reproducible methods for producing these models. Various techniques ... More
Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.
'Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single ... More