Versene Solution

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Citations & References (2)

Gibco™

Versene Solution

Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) isRead more

Catalog Number	Quantity
15040066	100 mL

Catalog number 15040066

Price (CNY)

253.00

Add to cart

Quantity:

100 mL

Price (CNY)

253.00

Add to cart

Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na₄) per liter of Phosphate Buffered Saline (PBS).

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco Versene Solution at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ChelatorsEDTA

Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485

Quantity100 mL

Shelf Life18 Months

Shipping ConditionRoom Temperature

ClassificationAnimal Origin-free

Product TypeCell Culture Dissociation Reagent

SterilitySterile-filtered

With AdditivesEDTA

Unit SizeEach

Contents & Storage

Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

Frequently asked questions (FAQs)

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Can I use EDTA or versene for passaging of stem cells?

Yes, you may use EDTA or versene. However, we do not recommend using dispase or collagenase as it can lead to differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is Gibco Versene Solution still the recommended dissociation reagent with Gibco Essential 8 Flex Medium?

Both Versene Solution (Cat. No. 15040066) and enzyme-mediated dissociation have been shown to be compatible passaging methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & References

Abstract

Decreased metallation and activity in subsets of mutant superoxide dismutases associated with familial amyotrophic lateral sclerosis.

Authors: Hayward Lawrence J; Rodriguez Jorge A; Kim Ji W; Tiwari Ashutosh; Goto Joy J; Cabelli Diane E; Valentine Joan Selverstone; Brown Robert H Jr;

Journal:J Biol Chem

PubMed ID:11854284

Over 90 different mutations in the gene encoding copper/zinc superoxide dismutase (SOD1) cause approximately 2% of amyotrophic lateral sclerosis (ALS) cases by an unknown mechanism. We engineered 14 different human ALS-related SOD1 mutants and obtained high yields of biologically metallated proteins from an Sf21 insect cell expression system. Both the ... More

Molecular determinants of receptor binding and signaling by the CX3C chemokine fractalkine.

Authors: Mizoue L S; Sullivan S K; King D S; Kledal T N; Schwartz T W; Bacon K B; Handel T M;

Journal:J Biol Chem

PubMed ID:11432858

Fractalkine/CX3CL1 is a membrane-tethered chemokine that functions as a chemoattractant and adhesion protein by interacting with the receptor CX3CR1. To understand the molecular basis for the interaction, an extensive mutagenesis study of fractalkine's chemokine domain was undertaken. The results reveal a cluster of basic residues (Lys-8, Lys-15, Lys-37, Arg-45, and ... More