Versene Solution - FAQs

查看更多产品信息 Versene Solution - FAQs (15040066)

8 个常见问题解答

你们提供哪些用于解离细胞的试剂？这些产品之间的主要区别是什么？

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

在Gibco Essential 8 Flex培养基中培养细胞时，我是否需要在传代过程中加入ROCK抑制剂？

Gibco Essential 8 Flex培养基与其他许多PSC培养基一样，在使用乙二胺四乙酸溶液成集落传代PSC细胞时，无需加入ROCK抑制剂。如果进行单细胞传代，我们则推荐添加Gibco RevitaCell添加剂（货号 A2644501）或其他ROCK抑制剂。

Gibco 乙二胺四乙酸溶液是有效兼容Gibco Essential 8 Flex培养基的推荐解离试剂么？

Gibco 乙二胺四乙酸溶液（Gibco Versene Solution，货号 15040066）与酶解离法均适用于传代操作。

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Can I use EDTA or versene for passaging of stem cells?

Yes, you may use EDTA or versene. However, we do not recommend using dispase or collagenase as it can lead to differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is Gibco Versene Solution still the recommended dissociation reagent with Gibco Essential 8 Flex Medium?

Both Versene Solution (Cat. No. 15040066) and enzyme-mediated dissociation have been shown to be compatible passaging methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.