I received a shipment of UltraPure 0.5M EDTA, pH 8.0 (Cat. No. 15575020), but it was stored below 15-30 degrees C over the weekend. Can you confirm this product will still be stable?
We do not have specific data for temperatures below 15 degrees C for UltraPure 0.5M EDTA, pH 8.0 (Cat. No. 15575020). However, as long as the product was not frozen, the performance should not be affected.
EDTA binds Mg2+ and Ca2+, while EGTA specifically binds to Ca2+. The use of EGTA may be favored in case where subsequent treatment with Mg2+ dependent enzymes is planned.
How does EDTA work?
EDTA reversibly binds Mg2+, Ca2+ as well as other divalent cations, thus inhibiting enyzmes that require these cations for activity.
引用和文献 (7)
引用和文献
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Nondestructive sampling of human skeletal remains yields ancient nuclear and mitochondrial DNA.
Authors:Bolnick DA, Bonine HM, Mata-Míguez J, Kemp BM, Snow MH, LeBlanc SA
Journal:Am J Phys Anthropol
PubMed ID:22183740
'Museum curators and living communities are sometimes reluctant to permit ancient DNA (aDNA) studies of human skeletal remains because the extraction of aDNA usually requires the destruction of at least some skeletal material. Whether these views stem from a desire to conserve precious materials or an objection to destroying ancestral ... More
Digital gene expression by tag sequencing on the illumina genome analyzer.
Authors:Morrissy S, Zhao Y, Delaney A, Asano J, Dhalla N, Li I, McDonald H, Pandoh P, Prabhu AL, Tam A, Hirst M, Marra M
Journal:Curr Protoc Hum Genet
PubMed ID:20373513
'This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag-seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ... More
DNA extraction from aged skeletal samples for STR typing by capillary electrophoresis.
Authors:Huel R, Amory S, Bilic A, Vidovic S, Jasaragic E, Parsons TJ
Journal:Methods Mol Biol
PubMed ID:22139661
STR analysis of DNA extracted from skeletal samples can play an important role in the identification of missing persons. Here we present a method for the extraction of DNA from skeletal samples involving complete demineralization and digestion of the sample, followed by purification by silica binding. This method, together with ... More
Lentiviral transduction and clonal selection of hESCs with endothelial-specific transgenic reporters.
Authors:James D, Zhan Q, Kloss C, Zaninovic N, Rosenwaks Z, Rafii S
Journal:Curr Protoc Stem Cell Biol
PubMed ID:21509935
Generation of vascular endothelial cells (EC) from human embryonic stem cells (hESC) is a vital component of cell-based strategies for treatment of cardiovascular disease. Before hESC-derived ECs can be administered in therapeutic modalities, however, chemically defined culture conditions must be developed that reproducibly and robustly induce vascular differentiation. One approach ... More