Nondestructive sampling of human skeletal remains yields ancient nuclear and mitochondrial DNA.
AuthorsBolnick DA, Bonine HM, Mata-Míguez J, Kemp BM, Snow MH, LeBlanc SA
JournalAm J Phys Anthropol
PubMed ID22183740
'Museum curators and living communities are sometimes reluctant to permit ancient DNA (aDNA) studies of human skeletal remains because the extraction of aDNA usually requires the destruction of at least some skeletal material. Whether these views stem from a desire to conserve precious materials or an objection to destroying ancestral ... More
Digital gene expression by tag sequencing on the illumina genome analyzer.
AuthorsMorrissy S, Zhao Y, Delaney A, Asano J, Dhalla N, Li I, McDonald H, Pandoh P, Prabhu AL, Tam A, Hirst M, Marra M
JournalCurr Protoc Hum Genet
PubMed ID20373513
'This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag-seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ... More
DNA extraction from aged skeletal samples for STR typing by capillary electrophoresis.
AuthorsHuel R, Amory S, Bilic A, Vidovic S, Jasaragic E, Parsons TJ
JournalMethods Mol Biol
PubMed ID22139661
STR analysis of DNA extracted from skeletal samples can play an important role in the identification of missing persons. Here we present a method for the extraction of DNA from skeletal samples involving complete demineralization and digestion of the sample, followed by purification by silica binding. This method, together with ... More
Lentiviral transduction and clonal selection of hESCs with endothelial-specific transgenic reporters.
AuthorsJames D, Zhan Q, Kloss C, Zaninovic N, Rosenwaks Z, Rafii S
JournalCurr Protoc Stem Cell Biol
PubMed ID21509935
Generation of vascular endothelial cells (EC) from human embryonic stem cells (hESC) is a vital component of cell-based strategies for treatment of cardiovascular disease. Before hESC-derived ECs can be administered in therapeutic modalities, however, chemically defined culture conditions must be developed that reproducibly and robustly induce vascular differentiation. One approach ... More
Production of p53 gene knockout rats by homologous recombination in embryonic stem cells.
AuthorsTong C, Li P, Wu NL, Yan Y, Ying QL
JournalNature
PubMed ID20703227
The use of homologous recombination to modify genes in embryonic stem (ES) cells provides a powerful means to elucidate gene function and create disease models. Application of this technology to engineer genes in rats has not previously been possible because of the absence of germline-competent ES cells in this species. ... More
The impact of pre-analytical processing on staining quality for H&E, dual hapten, dual color in situ hybridization and fluorescent in situ hybridization assays.
AuthorsBabic A, Loftin IR, Stanislaw S, Wang M, Miller R, Warren SM, Zhang W, Lau A, Miller M, Wu P, Padilla M, Grogan TM, Pestic-Dragovich L, McElhinny AS
JournalMethods
PubMed ID20807574
With the advent of personalized medicine, anatomic pathology-based molecular assays, including in situ hybridization (ISH) and mRNA detection tests, are performed routinely in many laboratories and have increased in their clinical importance and complexity. These assays require appropriately fixed tissue samples that preserve both nucleic acid targets and histomorphology to ... More