The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.
Authors Barendt Wendy M; Wright Stephen H;
JournalJ Biol Chem
PubMed ID11953440
'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human ... More
The role of polymer nanolayer architecture on the separation performance of anion-exchange membrane adsorbers: part II. DNA and virus separations.
AuthorsBhut BV, Weaver J, Carter AR, Wickramasinghe SR, Husson SM,
JournalBiotechnol Bioeng
PubMed ID21618476
'The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the role of polymer nanolayer architecture on DNA and virus binding. ... More
Spectrophotometric analysis of nucleic acids: oxygenation-dependent hyperchromism of DNA.
AuthorsDoshi R, Day PJ, Carampin P, Blanch E, Stratford IJ, Tirelli N,
JournalAnal Bioanal Chem
PubMed ID20169336
'The absorbance at 260 nm (A(260)) is ubiquitously used for nucleic acid quantification. We show that following oxygenation, DNA solutions experience alterations in both spectral properties (hyperchromism in the UV region, lambda(max) 260 nm) and DNA conformation. The spectral changes caused by oxygen-DNA complexation are stable for at least several ... More
A quantitative real-time polymerase chain reaction assay for the seagrass pathogen Labyrinthula zosterae.
'The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. ... More
Noncoding DNA in lipofection of HeLa cells-a few insights.
AuthorsSymens N, Rejman J, Lucas B, Demeester J, De Smedt SC, Remaut K,
JournalMol Pharm
PubMed ID23421924
'In cationic carrier-mediated gene delivery, the disproportional relationship between the quantity of delivered DNA and the amount of encoded protein produced is a well-known phenomenon. The numerous intracellular barriers which need to be overcome by pDNA to reach the nucleoplasm play a major role in it. In contrast to what ... More
Kinetics and thermodynamics of phenotype: unwinding and rewinding the nucleosome.
Chromatin endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo.
AuthorsGriesenbeck J, Wittner M, Charton R, Conconi A,
JournalMethods Mol Biol
PubMed ID22113284
In eukaryotes, multiple copies of ribosomal RNA (rRNA) genes co-exist in two different chromatin states: actively transcribed (nucleosome depleted) chromatin, and nontranscribed (nucleosomal) chromatin. The presence of two rRNA gene populations compromises the interpretation of analyses obtained by the standard biochemical methods that are used to study chromatin structure (e.g., ... More
Multiphasic DNA Adsorption to Silica Surfaces under Varying Buffer, pH, and Ionic Strength Conditions.
AuthorsVandeventer PE, Lin JS, Zwang TJ, Nadim A, Johal MS, Niemz A,
JournalJ Phys Chem B
PubMed ID22537288
Reversible interactions between DNA and silica are utilized in the solid phase extraction and purification of DNA from complex samples. Chaotropic salts commonly drive DNA binding to silica but inhibit DNA polymerase amplification. We studied DNA adsorption to silica using conditions with or without chaotropic salts through bulk depletion and ... More