Platinum™ Taq DNA 聚合酶(不含 DNA)
Platinum™ Taq DNA 聚合酶(不含 DNA)
Invitrogen™

Platinum™ Taq DNA 聚合酶(不含 DNA)

Invitrogen Platinum Taq DNA 聚合酶(不含 DNA)采用一种新型封闭一次性系统和严格的质量控制检测生产,以尽可能减少细菌和人 DNA 污染风险了解更多信息
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货号反应次数
15966005400 次反应
货号 15966005
价格(CNY)
2,902.00
Each
添加至购物车
反应次数:
400 次反应
请求批量或定制报价
价格(CNY)
2,902.00
Each
添加至购物车
Invitrogen Platinum Taq DNA 聚合酶(不含 DNA)采用一种新型封闭一次性系统和严格的质量控制检测生产,以尽可能减少细菌和人 DNA 污染风险。对于要求极高灵敏度且无因试剂传播的污染而产生假阳性结果的 PCR 应用,该酶是理想选择。该不含 DNA 酶提供与标准 Platinum Taq DNA 聚合酶一样,可提供高水平性能和批间一致性。

不含 DNA 的 Platinum Taq DNA 聚合酶:
•低 DNA 污染水平(≤0.01 拷贝细菌 gDNA/酶单位)经过认证
•使用无模板/仅使用试剂的 PCR 对照品时信号缺失,因此低丰度微生物的定性和定量检测较为可信
•与使用常规工艺生产的 Platinum Taq DNA 聚合酶的性能相同

Platinum Taq DNA 聚合酶(不含 DNA)是一种可与专有抗体形成复合物的重组 Taq 聚合酶,该专有抗体可在环境温度下阻断聚合酶活性,并在初始变性步骤后于 94°C 下解离。这种自动的“热启动”技术不但可以提高反应的灵敏度、特异性和得率,而且为反应体系可以在室温下进行配制提供了极大的便利性。正如 Taq DNA 聚合酶一样,Platinum Taq DNA 聚合酶(不含 DNA)具有不依赖模板的末端转移酶活性(其将 3′ 脱氧腺苷添加到产物末端),并具有 5′→3′ 核酸外切酶活性。

使用 Platinum Taq DNA 聚合酶(不含 DNA)从复杂基因组、病毒模板以及 RT-PCR 中扩增 DNA。它是进行 PCR 检测的最佳选择,可满足更高灵敏度和特异性要求,尽可能减少不确定或假阳性结果。更多详细信息,请访问 www.thermofisher.cn/dna-free

仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)1 X
产品规格管装
热启动内置热启动
反应次数400 次反应
突出端3'-A
聚合酶Platinum Taq DNA 聚合酶
产品类型Taq DNA 聚合酶
数量500 单位
反应形式分离组分
运输条件干冰
尺寸(最终产品)5 kb或更小
原始材料DNA
最大浓度5 U/μL
适用于(应用)Hot-start PCR
高 GC PCR 扩增效果
反应速度标准
Unit SizeEach
内容与储存
• Platinum Taq DNA 聚合酶(不含 DNA),100 µL
• 10X PCR 缓冲液 (-MgCl2)(不含 DNA),1.25 mL
• 50 mM MgCl2(不含 DNA),1 mL

储存在 -5 至 -30°C 非自动除霜冰箱中。

常见问题解答 (FAQ)

What is the unit concentration of Platinum Taq DNA Polymerase, DNA-free?

Platinum Taq DNA Polymerase, DNA-free is available in 5.0 U/µL enzyme concentration.

Can I assemble my PCR reactions with Platinum Taq DNA Polymerase, DNA-free at room temperature?

Yes. Due to stable antibody-mediated hot-start technology, Platinum Taq DNA Polymerase, DNA-free is highly stable. The PCR reactions can be assembled at room temperature without any loss of amplification specificity.

I am using Platinum Taq DNA Polymerase, DNA-free for PCR. "No-template controls" in my experiment are showing a background amplification signal. What should I do differently?

Amplification signal in “no-template controls” (NTC), also known as false-positive signal, or background amplification in “reagent-only control”, may arise from contaminating DNA that entered control reactions from the environment, from the researcher, or was introduced into the PCR via contaminated PCR reagents or consumables. To avoid contaminating DNA in your experiments, it is important to follow these recommendations:

- Be cautious to maintain a DNA-free environment during handling and opening the vials with DNA polymerase, PCR buffer, and MgCl2
- Ensure that all components of the PCR, like dNTPs, primers, probes, or water are free of contaminating DNA that can give false positive PCR results
- Avoid opening the tubes containing DNA-free reagents multiple times to minimize risk of DNA contamination
- Work in a UV-irradiated workstation
- Use certified DNA-free pipette tips and PCR consumables

Can I use the same cycling protocol that I use with standard Taq for PCR reactions with Platinum Taq DNA Polymerase, DNA-free?

PCR reactions with Platinum Taq DNA Polymerase, DNA-free can be run using the same cycling protocol as that for standard Taq.

Can I use Platinum Taq DNA Polymerase, DNA-free in qPCR?

Yes, Platinum Taq DNA Polymerase, DNA-free can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.