Platinum™ Taq DNA Polymerase, DNA-free, 500 Units - FAQs

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8 product FAQs found

What is the unit concentration of Platinum Taq DNA Polymerase, DNA-free?

Platinum Taq DNA Polymerase, DNA-free is available in 5.0 U/µL enzyme concentration.

Can I assemble my PCR reactions with Platinum Taq DNA Polymerase, DNA-free at room temperature?

Yes. Due to stable antibody-mediated hot-start technology, Platinum Taq DNA Polymerase, DNA-free is highly stable. The PCR reactions can be assembled at room temperature without any loss of amplification specificity.

I am using Platinum Taq DNA Polymerase, DNA-free for PCR. "No-template controls" in my experiment are showing a background amplification signal. What should I do differently?

Amplification signal in “no-template controls” (NTC), also known as false-positive signal, or background amplification in “reagent-only control”, may arise from contaminating DNA that entered control reactions from the environment, from the researcher, or was introduced into the PCR via contaminated PCR reagents or consumables. To avoid contaminating DNA in your experiments, it is important to follow these recommendations:

- Be cautious to maintain a DNA-free environment during handling and opening the vials with DNA polymerase, PCR buffer, and MgCl2
- Ensure that all components of the PCR, like dNTPs, primers, probes, or water are free of contaminating DNA that can give false positive PCR results
- Avoid opening the tubes containing DNA-free reagents multiple times to minimize risk of DNA contamination
- Work in a UV-irradiated workstation
- Use certified DNA-free pipette tips and PCR consumables

Can I use the same cycling protocol that I use with standard Taq for PCR reactions with Platinum Taq DNA Polymerase, DNA-free?

PCR reactions with Platinum Taq DNA Polymerase, DNA-free can be run using the same cycling protocol as that for standard Taq.

Can I use Platinum Taq DNA Polymerase, DNA-free in qPCR?

Yes, Platinum Taq DNA Polymerase, DNA-free can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.

What other PCR components do you recommend using with Platinum Taq DNA Polymerase, DNA-free?

To avoid false positive results, we recommend using Platinum Taq DNA Polymerase, DNA-free with 10 mM dNTP Mix (Cat. No. 18427088) and RT-PCR Grade Water (Cat. No. AM9935).

What are the purity requirements for Platinum Taq DNA Polymerase, DNA-free?

Platinum Taq DNA Polymerase, DNA-free is certified to contain low-DNA contamination level: ≤0.01 copy of bacterial gDNA per enzyme unit; ≤0.001 copy/enzyme unit of human gDNA; ≤0.01 copy/enzyme unit of plasmid DNA.

What is the difference between Platinum Taq DNA Polymerase and Platinum Taq DNA Polymerase, DNA-free?

Platinum Taq DNA Polymerase, DNA-free is manufactured using closed and single-use system technology to minimize DNA contamination risk. Platinum Taq DNA Polymerase, DNA-free provides the same high level of performance and lot-to-lot consistency that is expected for the standard Platinum Taq DNA Polymerase.