我们的UltraPure琼脂糖是标准熔点琼脂糖,适用于DNA和RNA片段的常规分离和分析,分离范围为500–23,000bp。UltraPure琼脂糖1000是特制的琼脂糖,为PCR片段和其它短DNA片段提供较高的分辨率。我们也提供UltraPure低熔点琼脂糖,是在65°C或更低的温度下分离10到1000 bp DNA片段的理想选择。
I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
引用和文献 (6)
引用和文献
Abstract
Co-injection of a targeted, reversibly masked endosomolytic polymer dramatically improves the efficacy of cholesterol-conjugated small interfering RNAs in vivo.
Authors:Wong SC, Klein JJ, Hamilton HL, Chu Q, Frey CL, Trubetskoy VS, Hegge J, Wakefield D, Rozema DB, Lewis DL
Journal:Nucleic Acid Ther
PubMed ID:23181701
'Effective in vivo delivery of small interfering (siRNA) has been a major obstacle in the development of RNA interference therapeutics. One of the first attempts to overcome this obstacle utilized intravenous injection of cholesterol-conjugated siRNA (chol-siRNA). Although studies in mice revealed target gene knockdown in the liver, delivery was relatively ... More
Relationship of IL-1 and TNF-a polymorphisms with Helicobacter pylori in gastric diseases in a Brazilian population.
Authors:Santos JC, Ladeira MS, Pedrazzoli J, Ribeiro ML
Journal:Braz J Med Biol Res
PubMed ID:22714811
'It is well known that the risk of development of gastric cancer (GC) in Helicobacter pylori-infected patients depends on several factors. Thus, the aim of this study was to investigate the effect of proinflammatory cytokine gene polymorphisms for IL-1ß, IL-1RN and TNF-a on the development of GC in a Brazilian ... More
Polymorphisms of the TLR2 and TLR4 genes are associated with risk of gastric cancer in a Brazilian population.
Authors:de Oliveira JG, Silva AE
Journal:World J Gastroenterol
PubMed ID:22468087
'To investigate toll-like receptor 2 (TLR2) -196 to -174 del, and TLR4 (+896A/G rs4986790 and +1196C/T rs4986791) polymorphisms at risk of chronic gastritis and gastric cancer in a Brazilian population and association of gastric lesions with risk factors such as smoking, alcohol intake and Helicobacter pylori infection.' ... More
Transcriptome characterization and gene expression of Epinephelus spp in endoplasmic reticulum stress-related pathway during betanodavirus infection in vitro.
Authors:Lu MW, Ngou FH, Chao YM, Lai YS, Chen NY, Lee FY, Chiou PP
Journal:BMC Genomics
PubMed ID:23170826
'Grouper (Epinephelus spp) is an economically important fish species worldwide. However, viral pathogens such as nervous necrosis virus (NNV) have been causing severe infections in the fish, resulting in great loss in the grouper aquaculture industry. Yet, the understanding of the molecular mechanisms underlying the pathogenicity of NNV is still ... More
A non-exothermic cell-embedding tissue-mimicking material for studies of ultrasound-induced hyperthermia and drug release.
Authors:Mylonopoulou E, Bazán-Peregrino M, Arvanitis CD, Coussios CC
Journal:Int J Hyperthermia
PubMed ID:23406389
'The present study aims to create and characterise a cell-embedding tissue-mimicking material (TMM) that has thermal and acoustic properties similar to liver tissue, in order to enable study and optimisation of protocols for ultrasound-induced hyperthermia and drug delivery.' ... More