我们的UltraPure琼脂糖是标准熔点琼脂糖,适用于DNA和RNA片段的常规分离和分析,分离范围为500–23,000bp。UltraPure琼脂糖1000是特制的琼脂糖,为PCR片段和其它短DNA片段提供较高的分辨率。我们也提供UltraPure低熔点琼脂糖,是在65°C或更低的温度下分离10到1000 bp DNA片段的理想选择。
I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
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