Co-injection of a targeted, reversibly masked endosomolytic polymer dramatically improves the efficacy of cholesterol-conjugated small interfering RNAs in vivo.
AuthorsWong SC, Klein JJ, Hamilton HL, Chu Q, Frey CL, Trubetskoy VS, Hegge J, Wakefield D, Rozema DB, Lewis DL
JournalNucleic Acid Ther
PubMed ID23181701
'Effective in vivo delivery of small interfering (siRNA) has been a major obstacle in the development of RNA interference therapeutics. One of the first attempts to overcome this obstacle utilized intravenous injection of cholesterol-conjugated siRNA (chol-siRNA). Although studies in mice revealed target gene knockdown in the liver, delivery was relatively ... More
Relationship of IL-1 and TNF-a polymorphisms with Helicobacter pylori in gastric diseases in a Brazilian population.
AuthorsSantos JC, Ladeira MS, Pedrazzoli J, Ribeiro ML
JournalBraz J Med Biol Res
PubMed ID22714811
'It is well known that the risk of development of gastric cancer (GC) in Helicobacter pylori-infected patients depends on several factors. Thus, the aim of this study was to investigate the effect of proinflammatory cytokine gene polymorphisms for IL-1ß, IL-1RN and TNF-a on the development of GC in a Brazilian ... More
Polymorphisms of the TLR2 and TLR4 genes are associated with risk of gastric cancer in a Brazilian population.
Authorsde Oliveira JG, Silva AE
JournalWorld J Gastroenterol
PubMed ID22468087
'To investigate toll-like receptor 2 (TLR2) -196 to -174 del, and TLR4 (+896A/G rs4986790 and +1196C/T rs4986791) polymorphisms at risk of chronic gastritis and gastric cancer in a Brazilian population and association of gastric lesions with risk factors such as smoking, alcohol intake and Helicobacter pylori infection.' ... More
Transcriptome characterization and gene expression of Epinephelus spp in endoplasmic reticulum stress-related pathway during betanodavirus infection in vitro.
AuthorsLu MW, Ngou FH, Chao YM, Lai YS, Chen NY, Lee FY, Chiou PP
JournalBMC Genomics
PubMed ID23170826
'Grouper (Epinephelus spp) is an economically important fish species worldwide. However, viral pathogens such as nervous necrosis virus (NNV) have been causing severe infections in the fish, resulting in great loss in the grouper aquaculture industry. Yet, the understanding of the molecular mechanisms underlying the pathogenicity of NNV is still ... More
A non-exothermic cell-embedding tissue-mimicking material for studies of ultrasound-induced hyperthermia and drug release.
AuthorsMylonopoulou E, Bazán-Peregrino M, Arvanitis CD, Coussios CC
JournalInt J Hyperthermia
PubMed ID23406389
'The present study aims to create and characterise a cell-embedding tissue-mimicking material (TMM) that has thermal and acoustic properties similar to liver tissue, in order to enable study and optimisation of protocols for ultrasound-induced hyperthermia and drug delivery.' ... More
The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells.
AuthorsLindqvist A, Manders D, Word RA
JournalBMC Res Notes
PubMed ID23363446
Accurate measurements of mRNA expression levels in tissues or cells are crucially dependent on the use of relevant reference genes for normalization of data. In this study we used quantitative real-time PCR and two Excel-based applets (geNorm and BestKeeper) to determine the best reference genes for quantification of target gene ... More