N-2 添加剂 (100X)
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N-2 添加剂 (100X)
Gibco™

N-2 添加剂 (100X)

N-2 添加剂基于 Bottenstein’s N-1 配方，是一种化学成分确定的无血清添加剂。推荐用于支持源自外周神经系统和中枢神经系统的原代神经母细胞瘤及有丝分裂后神经元的生长和表达。N-2 添加剂作为 100X 浓缩液提供，可与补充生长因子了解更多信息
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货号数量
175020485 mL
1750200150 mL
货号 17502048
价格（CNY)
1,092.70
飞享价
Ends: 31-Dec-2025
1,586.00
共减 493.30 (31%)
Each
添加至购物车
数量:
5 mL
价格（CNY)
1,092.70
飞享价
Ends: 31-Dec-2025
1,586.00
共减 493.30 (31%)
Each
添加至购物车
N-2 添加剂基于 Bottenstein’s N-1 配方，是一种化学成分确定的无血清添加剂。推荐用于支持源自外周神经系统和中枢神经系统的原代神经母细胞瘤及有丝分裂后神经元的生长和表达。N-2 添加剂作为 100X 浓缩液提供，可与补充生长因子（如 bFGF 和 EGF）后的 Neurobasal 培养基配合使用。它也可以与 DMEM 配合使用。

有关使用 N-2 添加剂的成熟实验方案，请访问本公司的 Gibco 神经生物学实验方案手册。

如需查看使用 Gibco 神经生物学产品的出版物，请访问本公司的 Gibco CellCite。

仅供科研使用。不可用于诊断程序。
规格
细胞类型神经细胞
最大浓度100 X
培养类型哺乳动物细胞培养
数量5 mL
血清水平无血清
有效期18 个月
运输条件干冰
经过测试内毒素、性能、无菌性
分类无血清
形式液体
产品类型细胞培养添加剂
无菌无菌
pH6 - 8
Unit SizeEach
内容与储存
储存在冰箱（-5 至 -20°C）中并避光。

常见问题解答 (FAQ)

在培养神经细胞的过程中，我可在Neurobasal培养基中加入哪些添加剂？

做为一般性的参考方案，我们推荐您使用Gibco B-27添加剂来培养神经干细胞，海马及其他类型的CNS神经元。

-对于来自PNS和CNS中的神经母细胞瘤或有丝分裂后的神经元，可添加Gibco N-2添加剂。
-对于原代胶质细胞（星形胶质细胞）或具有星形胶质细胞表型的肿瘤细胞系（星形胶质细胞和胶质细胞瘤），或少突胶质细胞，可使用Gibco G-5添加剂。

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference between the Gibco N-2 Supplement formulation from Thermo Fisher and the N-2 Supplement formulation from Global Stem?

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I filter the N-2 Supplement?

The N-2 Supplement (100X) solution can be filtered. We do not recommend filtering the medium once N-2 has been added at a final concentration of 1X.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition and shelf life for N-2 Supplement (100X) (Cat. No. 17502001 or 17502048)?

The shelf life for N-2 Supplement (100X) is 18 months from the date of manufacture when stored at the recommended storage condition of -5 to -20 degrees C, protected from light. The expiration date should be listed on the product label as well as on the COA for the lot of the supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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