N-2 Supplement (100X), 5 mL - FAQs

View additional product information for N-2 Supplement (100X) - FAQs (17502001, 17502048)

6 product FAQs found

在培养神经细胞的过程中，我可在Neurobasal培养基中加入哪些添加剂？

做为一般性的参考方案，我们推荐您使用Gibco B-27添加剂来培养神经干细胞，海马及其他类型的CNS神经元。

-对于来自PNS和CNS中的神经母细胞瘤或有丝分裂后的神经元，可添加Gibco N-2添加剂。
-对于原代胶质细胞（星形胶质细胞）或具有星形胶质细胞表型的肿瘤细胞系（星形胶质细胞和胶质细胞瘤），或少突胶质细胞，可使用Gibco G-5添加剂。

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference between the Gibco N-2 Supplement formulation from Thermo Fisher and the N-2 Supplement formulation from Global Stem?

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I filter the N-2 Supplement?

The N-2 Supplement (100X) solution can be filtered. We do not recommend filtering the medium once N-2 has been added at a final concentration of 1X.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition and shelf life for N-2 Supplement (100X) (Cat. No. 17502001 or 17502048)?

The shelf life for N-2 Supplement (100X) is 18 months from the date of manufacture when stored at the recommended storage condition of -5 to -20 degrees C, protected from light. The expiration date should be listed on the product label as well as on the COA for the lot of the supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which supplement can I add to Neurobasal Medium to culture my neural cells?

As a general guideline, we recommend using Gibco B-27 Plus Supplement to culture neural stem cells, hippocampal, and other CNS neurons.

- For neuroblastomas, or post-mitotic neurons from both PNS and CNS, Gibco N-2 Supplement can be used.
- For primary glial cells (astrocytes) or tumor cell lines of astrocytic phenotype (astrocytes and gliomas), or oligodendrocytes, Gibco G-5 Supplement can be used.