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引用和文献 (6)
Invitrogen™
S1 核酸酶
S1 核酸酶是一种单链特异性核酸内切酶，可水解单链 RNA 或 DNA 为 5´ 单核苷酸。该酶将水解双螺旋 DNA了解更多信息
| 货号 | 数量 |
|---|---|
| 18001016 | 20,000 U |
货号 18001016
价格（CNY)
2,456.00
Each
数量:
20,000 U
价格（CNY)
2,456.00
Each
S1 核酸酶是一种单链特异性核酸内切酶，可水解单链 RNA 或 DNA 为 5´ 单核苷酸。该酶将水解双螺旋 DNA 中的单链区域，如环和裂隙。S1 核酸酶在 65°C 下稳定储存。
应用：核酸酶绘图技术 (1,2)。去除双链 DNA 中的单链区域 (3)。外显子 III 有序测序 (4)。
来源：从米曲霉中分离得到。
性能和质量检测：双链特异性脱氧核糖核酸酶和磷酸酶测定。
单元定义： 一个单元可在 37°C 下 1 min 内将 1μg 变性 DNA 水解为酸溶性物质。
单元反应条件：30 mM 醋酸钠（pH 值 4.6）、50 mM NaCl、1 mM 醋酸锌、0.5 mg/mL 热变性 DNA、5% (v/v) 甘油以及 0.5 mL 酶，37°C 下持续 10 min。
应用：核酸酶绘图技术 (1,2)。去除双链 DNA 中的单链区域 (3)。外显子 III 有序测序 (4)。
来源：从米曲霉中分离得到。
性能和质量检测：双链特异性脱氧核糖核酸酶和磷酸酶测定。
单元定义： 一个单元可在 37°C 下 1 min 内将 1μg 变性 DNA 水解为酸溶性物质。
单元反应条件：30 mM 醋酸钠（pH 值 4.6）、50 mM NaCl、1 mM 醋酸锌、0.5 mg/mL 热变性 DNA、5% (v/v) 甘油以及 0.5 mL 酶，37°C 下持续 10 min。
仅供科研使用。不可用于诊断程序。
规格
产品类型核酸酶
数量20,000 U
运输条件经批准可置于湿冰或干冰上运输
Unit SizeEach
内容与储存
S1 核酸酶配有一瓶 10X S1 核酸酶缓冲液 [300mM 醋酸钠（pH 值 4.6）、10mM 醋酸锌、50% (v/v) 甘油]、一瓶稀释缓冲液、一瓶 3M NaCl。保存于 -20°C。
常见问题解答 (FAQ)
What are the activities and applications of Exonuclease III, Mung Bean Nuclease, and S1 Nuclease?
引用和文献 (6)
引用和文献
Abstract
Isolation and characterization of the human cytochrome P450 CYP1B1 gene.
Journal:J Biol Chem
PubMed ID:8910454
'Previously, we identified a novel human cytochrome P450 cDNA that is inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and represents the first member of a new subfamily designated cytochrome P4501B1 (CYP1B1; Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W.,
Characterization of the 5' flanking region of the human D1A dopamine receptor gene [published erratum appears in Proc Natl Acad Sci U S A 1992 May 1;89(9):4220]
Journal:Proc Natl Acad Sci U S A
PubMed ID:1557411
'To study how the expression of the D1A dopamine receptor gene is regulated, a human genomic clone was isolated by using a rat cDNA as probe. A 2.3-kilobase genomic fragment spanning -2571 through -236 relative to the adenosine of the first methionine codon was sequenced. The gene has an intron
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
Journal:Cell
PubMed ID:6288265
The single-stranded activity of S1-nuclease cleaves globin chromatin in red cell nuclei in specific regions. The cleavages are observed only in tissues in which the globin genes are active, and they
Heteroduplex cleavage analysis using S1 nuclease.
Journal:Biotechniques
PubMed ID:10407654
Authors describe a version of heteroduplex cleavage analysis of PCR products based on the ability of S1 nuclease to cleave base pair mismatches in DNA/DNA heteroduplexes. The procedure requires very little sample, no purification, a short 30 minute incubation and can be visualized by agarose gel in less than
High-density fiber-optic genosensor microsphere array capable of zeptomole detection limits.
Journal:Anal Chem
PubMed ID:11985315
The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-microm-diameter microspheres on the distal face of a 500-microm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the