Isolation and characterization of the human cytochrome P450 CYP1B1 gene.
Authors Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;
JournalJ Biol Chem
PubMed ID8910454
'Previously, we identified a novel human cytochrome P450 cDNA that is inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and represents the first member of a new subfamily designated cytochrome P4501B1 (CYP1B1; Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W., ... More
Characterization of the 5' flanking region of the human D1A dopamine receptor gene [published erratum appears in Proc Natl Acad Sci U S A 1992 May 1;89(9):4220]
'To study how the expression of the D1A dopamine receptor gene is regulated, a human genomic clone was isolated by using a rat cDNA as probe. A 2.3-kilobase genomic fragment spanning -2571 through -236 relative to the adenosine of the first methionine codon was sequenced. The gene has an intron ... More
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
Authors Larsen A; Weintraub H;
JournalCell
PubMed ID6288265
The single-stranded activity of S1-nuclease cleaves globin chromatin in red cell nuclei in specific regions. The cleavages are observed only in tissues in which the globin genes are active, and they ... More
Heteroduplex cleavage analysis using S1 nuclease.
Authors Howard J T; Ward J; Watson J N; Roux K H;
JournalBiotechniques
PubMed ID10407654
Authors describe a version of heteroduplex cleavage analysis of PCR products based on the ability of S1 nuclease to cleave base pair mismatches in DNA/DNA heteroduplexes. The procedure requires very little sample, no purification, a short 30 minute incubation and can be visualized by agarose gel in less than ... More
High-density fiber-optic genosensor microsphere array capable of zeptomole detection limits.
Authors Epstein Jason R; Lee Myoyong; Walt David R;
JournalAnal Chem
PubMed ID11985315
The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-microm-diameter microspheres on the distal face of a 500-microm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the ... More
In vitro selection of external guide sequences for directing RNase P-mediated inhibition of viral gene expression.
AuthorsZhou T, Kim J, Kilani AF, Kim K, Dunn W, Jo S, Nepomuceno E, Liu F.
JournalJ Biol Chem
PubMed ID12050148
External guide sequences (EGSs) are small RNA molecules that bind to a target mRNA, form a complex resembling the structure of a tRNA, and render the mRNA susceptible to hydrolysis by RNase P, a tRNA processing enzyme. An in vitro selection procedure was used to select EGSs that direct human ... More