Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.
'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression ... More
De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1.
Authors Chalfant Charles E; Rathman Kristin; Pinkerman Ryan L; Wood Rachel E; Obeid Lina M; Ogretmen Besim; Hannun Yusuf A;
JournalJ Biol Chem
PubMed ID11801602
'In a recent study, we showed that ceramide induces the dephosphorylation of SR proteins, a family of protein factors that regulate alternative splicing. In this study, the regulation of the alternative processing of pre-mRNA of both caspase 9 and Bcl-x(L) was examined in response to ceramide. Treatment of A549 lung ... More
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
AuthorsHopfer U, Hopfer H, Jablonski K, Stahl RA, Wolf G,
JournalJ Biol Chem
PubMed ID16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases ... More
High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.
Authors Bao Paul; Frutos Anthony G; Greef Charles; Lahiri Joydeep; Muller Uwe; Peterson Todd C; Warden Laurence; Xie Xinying;
JournalAnal Chem
PubMed ID11985309
The application of resonance light scattering (RLS) particles for high-sensitivity detection of DNA hybridization on cDNA microarrays is demonstrated. Arrays composed of approximately 2000 human genes ( ... More
mRNA decay is rapidly induced after spore germination of Saccharomyces cerevisiae.
Spores from the yeast Saccharomyces cerevisiae can germinate and resume their vegetative growth when placed in favorable conditions. Biochemical studies on germination have been limited by the difficulty of obtaining a pure population of spores germinating synchronously. Here, we report that spores can be purified and sorted according to their ... More
The murine beta-globin locus control region regulates the rate of transcription but not the hyperacetylation of histones at the active genes.
Authors Schübeler D; Groudine M; Bender M A;
JournalProc Natl Acad Sci U S A
PubMed ID11553791
Locus control regions (LCRs) are defined by their ability to confer high-level tissue-specific expression to linked genes in transgenic assays. Previously, we reported that, at its native site, the murine beta-globin LCR is required for high-level beta-globin gene expression, but is not required to initiate an open chromatin conformation of ... More