Modified gateway system for double shRNA expression and Cre/lox based gene expression.
AuthorsRadulovich N, Leung L, Tsao MS,
JournalBMC Biotechnol
PubMed ID21418658
'The growing need for functional studies of genes has set the stage for the development of versatile tools for genetic manipulations. Aiming to provide tools for high throughput analysis of gene functions, we have developed a modified short hairpin RNA (shRNA) and gene expression system based on Gateway Technology. The ... More
Multicolor and electron microscopic imaging of connexin trafficking.
Authors Gaietta Guido; Deerinck Thomas J; Adams Stephen R; Bouwer James; Tour Oded; Laird Dale W; Sosinsky Gina E; Tsien Roger Y; Ellisman Mark H;
JournalScience
PubMed ID11964472
'Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy. Here we used this approach to show that newly synthesized connexin43 was transported predominantly in ... More
Studies of the properties of human origin recognition complex and its Walker A motif mutants.
The eukaryotic six-subunit origin recognition complex (ORC) governs the initiation site of DNA replication and formation of the prereplication complex. In this report we describe the isolation of the wild-type Homo sapiens (Hs)ORC and variants containing a Walker A motif mutation in the Orc1, Orc4, or Orc5 subunit using the ... More
Requirement for either a host- or pectin-induced pectate lyase for infection of pisum sativum by nectriahematococca.
AuthorsRogers LM, Kim YK, Guo W, Gonzalez-Candelas L, Li D, Kolattukudy PE
JournalProc Natl Acad Sci U S A
PubMed ID10931947
Fungal pathogens usually have multiple genes that encode extracellular hydrolytic enzymes that may degrade the physical barriers in their hosts during the invasion process. Nectria hematococca, a plant pathogen, has two inducible pectate lyase (PL) genes (pel) encoding PL that can help degrade the carbohydrate barrier in the host. pelA ... More
UDP-glucuronate Decarboxylase, a Key Enzyme in Proteoglycan Synthesis. CLONING, CHARACTERIZATION, AND LOCALIZATION.
Authors Moriarity John L; Hurt K Joseph; Resnick Adam C; Storm Phillip B; Laroy Wouter; Schnaar Ronald L; Snyder Solomon H;
JournalJ Biol Chem
PubMed ID11877387
UDP-glucuronate decarboxylase (UGD) catalyzes the formation of UDP-xylose from UDP-glucuronate. UDP-xylose is then used to initiate glycosaminoglycan biosynthesis on the core protein of proteoglycans. In a yeast two-hybrid screen with the protein kinase Akt (protein kinase B), we detected interactions with a novel sequence, which we cloned and expressed. The ... More
The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Authors Frankel Adam; Yadav Neelu; Lee Jaeho; Branscombe Tina L; Clarke Steven; Bedford Mark T;
JournalJ Biol Chem
PubMed ID11724789
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In searching the human genome for protein arginine N-methyltransferase (PRMT) family members, a novel gene has been found on chromosome 1 that ... More
Mouse DTEF-1 (ETFR-1, TEF-5) Is a Transcriptional Activator in alpha 1-Adrenergic Agonist-stimulated Cardiac Myocytes.
Authors Maeda Tomoji; Mazzulli Joseph R; Farrance Iain K G; Stewart Alexandre F R;
JournalJ Biol Chem
PubMed ID11986313
alpha(1)-Adrenergic signaling in cardiac myocytes activates the skeletal muscle alpha-actin gene through an MCAT cis-element, the binding site of the transcriptional enhancer factor-1 (TEF-1) family of transcription factors. TEF-1 accounts for more than 85% of the MCAT binding activity in neonatal rat cardiac myocytes. Other TEF-1 family members account for ... More