ElectroMAX™ DH10B 细胞
ElectroMAX™ DH10B 细胞
引用和文献 (9)
Invitrogen™

ElectroMAX™ DH10B 细胞

ElectroMAX DH10 B 细胞是电转感受态大肠杆菌细胞，可提供极高的转化效率，达到 >1 x 1010 cfu/μg 质粒 DNA了解更多信息
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货号数量
182900155 x 100μL
货号 18290015
价格（CNY)
3,026.00
Each
添加至购物车
数量:
5 x 100μL
价格（CNY)
3,026.00
Each
添加至购物车
ElectroMAX DH10 B 细胞是电转感受态大肠杆菌细胞，可提供极高的转化效率，达到 >1 x 1010 cfu/μg 质粒 DNA。ElectroMAX DH10B 细胞适用于需要高转化效率的应用，如 cDNA 或 gDNA 文库构建。ElectroMAX DH10B 细胞可提供：

•高效转化使克隆最大化
• 用于 cDNA 或 gDNA 克隆功能的增强型遗传标记

极高转化效率
ElectroMAX DH10 B 细胞可提供极高的转化效率，可在 20 μL 反应中达到 >1 x 1010 转化体/μg 对照 DNA。这些高转化效率使 ElectroMAX DH10B 细胞适用于高效克隆原核和真核基因组 DNA 以及从真核基因组中高效地挽救质粒。ElectroMAX DH10B 细胞也适于构建基因库、使用质粒衍生载体生成 cDNA 文库以及用于存在有限 DNA 量的情况。

注：需要一种能够产生 16 kV/cm 场强的高电压电穿孔设备。

通用克隆功能
ElectroMAX DH10 B 细胞具有以下特点：

•Φ80lacZΔM15 标志物提供了 β-半乳糖苷酶基因的 α-互补，可在含有 X-gal 或 Bluo-gal 的琼脂平板上进行蓝白斑筛选
• mcrA 基因分型标志物和 mcrBC、mrr 缺失可克隆含有甲基胞嘧啶和甲基腺嘌呤的 DNA
• endA1 突变可提高质粒得率和数量

基因型：F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

查找您需要的细胞株和规格
DH10B 细胞具有电转感受态和化学感受态规格。我们的 ElectroMax DH10B T1R 细胞和 MegaX DH10B T1R 电转感受态细胞可提供耐受 T1 和 T5 噬菌体的优势。
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌Yes (Streptomycin)
蓝色/白色筛查是
是否可克隆甲基化 DNA是
克隆不稳定 DNA不适合克隆不稳定DNA
是否含 F' 附加体缺乏 F’附加体
高通量能力不兼容高通量应用（手动）
提高质粒质量是
质粒可用于 > 20 kb 质粒
制备无甲基化 DNA不适合制备未甲基化DNA
产品线DH10B, ElectroMAX
产品类型感受态细胞
数量5 x 100μL
减少克隆重组现象是
运输条件干冰
T1 噬菌体 - 抗性 (tonA)否
转化效率级别高效率 (> 10^9 cfu⁄μg)
产品规格One Shot
种属大肠杆菌
Unit SizeEach
内容与储存
包含：
• ElectroMAX DH10B 细胞：5 样本瓶、每瓶 100 μL
•pUC19 DNA (10 pg/μL)：1 个样品瓶，50μ L
• S.O.C.培养基：2 瓶，各 6 ml

感受态细胞在 -80°C 下储存。pUC19 DNA 在 -20°C 下储存。SOC 培养基在 4°C 或室温下储存。

引用和文献 (9)

引用和文献
Abstract
Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.
Authors:Richman SA, Kranz DM, Stone JD,
Journal:Methods Mol Biol
PubMed ID:19159105
'Over the past two decades, the field of biosensors has been developing fast, portable, and convenient detection tools for various molecules of interest, both biological and environmental. Although much attention is paid to the transduction portion of the sensor, the actual bioreceptor that binds the ligand is equally critical. Tight, ... More
Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice.
Authors: Matesic L E; Yip R; Reuss A E; Swing D A; O'Sullivan T N; Fletcher C F; Copeland N G; Jenkins N A;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504925
'The d, ash, and ln coat color mutations provide a unique model system for the study of vesicle transport in mammals. All three mutant loci encode genes that are required for the polarized transport of melanosomes, the specialized, pigment-containing organelles of melanocytes, to the neighboring keratinocytes and eventually into coat ... More
Association of syncoilin and desmin: linking intermediate filament proteins to the dystrophin-associated protein complex.
Authors: Poon Ellen; Howman Emily V; Newey Sarah E; Davies Kay E;
Journal:J Biol Chem
PubMed ID:11694502
'We recently identified a novel protein called syncoilin, a putative intermediate filament protein that interacts with alpha-dystrobrevin, a member of the dystrophin-associated protein complex. Syncoilin is found at the neuromuscular junction, sarcolemma, and Z-lines and is thought to be important for muscle fiber integrity. Based on the similar protein structure ... More
Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling.
Authors:Angelastro JM, Klimaschewski L, Tang S, Vitolo OV, Weissman TA, Donlin LT, Shelanski ML, Greene LA
Journal:Proc Natl Acad Sci U S A
PubMed ID:10984536
'Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of ... More
Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit.
Authors: Otten Linda G; Sio Charles F; Vrielink Johanna; Cool Robbert H; Quax Wim J;
Journal:J Biol Chem
PubMed ID:12198140
'Using directed evolution, we have selected an adipyl acylase enzyme that can be used for a one-step bioconversion of adipyl-7-aminodesacetoxycephalosporanic acid (adipyl-7-ADCA) to 7-ADCA, an important compound for the synthesis of semisynthetic cephalosporins. The starting point for the directed evolution was the glutaryl acylase from Pseudomonas SY-77. The gene fragment ... More
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