'Over the past two decades, the field of biosensors has been developing fast, portable, and convenient detection tools for various molecules of interest, both biological and environmental. Although much attention is paid to the transduction portion of the sensor, the actual bioreceptor that binds the ligand is equally critical. Tight, ... More
Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice.
Authors Matesic L E; Yip R; Reuss A E; Swing D A; O'Sullivan T N; Fletcher C F; Copeland N G; Jenkins N A;
JournalProc Natl Acad Sci U S A
PubMed ID11504925
'The d, ash, and ln coat color mutations provide a unique model system for the study of vesicle transport in mammals. All three mutant loci encode genes that are required for the polarized transport of melanosomes, the specialized, pigment-containing organelles of melanocytes, to the neighboring keratinocytes and eventually into coat ... More
Association of syncoilin and desmin: linking intermediate filament proteins to the dystrophin-associated protein complex.
Authors Poon Ellen; Howman Emily V; Newey Sarah E; Davies Kay E;
JournalJ Biol Chem
PubMed ID11694502
'We recently identified a novel protein called syncoilin, a putative intermediate filament protein that interacts with alpha-dystrobrevin, a member of the dystrophin-associated protein complex. Syncoilin is found at the neuromuscular junction, sarcolemma, and Z-lines and is thought to be important for muscle fiber integrity. Based on the similar protein structure ... More
Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling.
AuthorsAngelastro JM, Klimaschewski L, Tang S, Vitolo OV, Weissman TA, Donlin LT, Shelanski ML, Greene LA
JournalProc Natl Acad Sci U S A
PubMed ID10984536
'Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of ... More
Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit.
Authors Otten Linda G; Sio Charles F; Vrielink Johanna; Cool Robbert H; Quax Wim J;
JournalJ Biol Chem
PubMed ID12198140
'Using directed evolution, we have selected an adipyl acylase enzyme that can be used for a one-step bioconversion of adipyl-7-aminodesacetoxycephalosporanic acid (adipyl-7-ADCA) to 7-ADCA, an important compound for the synthesis of semisynthetic cephalosporins. The starting point for the directed evolution was the glutaryl acylase from Pseudomonas SY-77. The gene fragment ... More
bcn-1 Element-dependent activation of the laminin gamma 1 chain gene by the cooperative action of transcription factor E3 (TFE3) and Smad proteins.
'Laminin is a major component of the extracellular matrix. The laminin gamma1 chain is the least variant component of the laminin heterotrimeric assembly. The laminin gamma1 chain gene (LAMC1) expression is induced by several factors, including transforming growth factor-beta (TGF-beta). LAMC1 promoter contains a highly conserved transcriptional element, bcn-1. We ... More
Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy.
Subtraction suppression hybridization was used with high throughput screening to identify transcripts of genes that are differentially expressed in nasal epithelium following lesioning of the olfactory bulb, termed bulbectomy. We isolated the rat homologue of plunc, a murine gene highly expressed in lung and nasopharyngeal regions, by this method. Rat ... More
ADAMTS4 cleaves at the aggrecanase site (Glu373-Ala374) and secondarily at the matrix metalloproteinase site (Asn341-Phe342) in the aggrecan interglobular domain.
Authors Westling Jennifer; Fosang Amanda J; Last Karena; Thompson Vivian P; Tomkinson Kathy N; Hebert Tracy; McDonagh Thomas; Collins-Racie Lisa A; LaVallie Edward R; Morris Elisabeth A; Sandy John D;
JournalJ Biol Chem
PubMed ID11854269
Two major proteolytic cleavages, one at NITEGE(373)/A(374)RGSVI and the other at VDIPEN(341)/F(342)FGVGG, have been shown to occur in vivo within the interglobular domain of aggrecan. The Glu(373)-Ala(374) site is cleaved in vitro by aggrecanase-1 (ADAMTS4) and aggrecanase-2 (ADAMTS5), whereas the other site, at Asn(341)-Phe(342), is efficiently cleaved by matrix metalloproteinases ... More
Construction and characterization of a large-fragment chicken bacterial artificial chromosome library.
Authors Zimmer R; Verrinder Gibbins A M;
JournalGenomics
PubMed ID9192841
A large-insert bacterial artificial chromosome (BAC) library has been constructed from male chicken genomic DNA using the new pBeloBAC11 vector. The library was prepared in two parts, such that two-thirds of the BAC library (2976 clones) had an average insert size of 490 kb (80 clones analyzed), after optimization of ... More