dNTP Mix (10 mM ea), 100 μL - FAQs

查看更多产品信息 dNTP Mix (10 mM ea) - FAQs (18427088, 18427089, 18427013)

5 个常见问题解答

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions

Why does the annealing temperature have to be above 55 degrees C, when using the GeneAmp PCR Carryover Prevention kit (Cat. No. N808-0068) or any kit containing AmpErase UNG (Cat. No. N808-0096)?

Uracil-N-glycosylase (UNG) has activity in the temperature range of 4 degrees C to 55 degrees C, and is not completely inactivated by incubation at 95 degrees C. It is recommended that the annealing temperature be at or above 55 degrees C when using this enzyme with any of our PCR or Sequence Detection Systems kits to avoid possible degradation of newly synthesized dU-containing PCR products by residual UNG activity. For this reason, it is also recommended that the post-PCR soak be maintained at 72 degrees C (instead of 4 degrees C) if analysis of the PCR product will be performed using a gel-based method.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Describe the amplified product produced by the control reagents in the GeneAmp Gold PCR Reagent Kit with Multiplex Control (Cat. No. 4312778).

The control primers generate a 300 and a 500 bp amplicon from the lambda control DNA. The Lambda Multiplex Control primers, as well as the Lambda Control DNA are provided in this kit.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.