AminoLink™ Plus 微固定试剂盒,0.1 mL
AminoLink™ Plus 微固定试剂盒,0.1 mL
Thermo Scientific™

AminoLink™ Plus 微固定试剂盒,0.1 mL

The Thermo Scientific AminoLink Plus Micro Immobilization Kit enables covalent attachment of amine-containing ligands for use in antibody purification and了解更多信息
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货号数量
2047510 个色谱柱试剂盒
货号 20475
价格(CNY)
8,554.00
Each
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数量:
10 个色谱柱试剂盒
请求批量或定制报价
价格(CNY)
8,554.00
Each
添加至购物车
The Thermo Scientific AminoLink Plus Micro Immobilization Kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

Features of the AminoLink Plus Micro Immobilization Kit:

Optimized for small scale—ideal for coupling small amounts (25 to 250 μg) of purified antibodies, proteins and other amine-containing ligands for purification of specific antibodies from crude serum
High-performance affinity resin—covalent attachment of the protein to AminoLink Plus Coupling Resin results in a leach-resistant affinity support
Efficient coupling chemistry—immobilization efficiency greater than 80% (as measured with 4 hour coupling reaction using mammalian antibodies)
Fast and easy to use—perform wash and elution steps using a microcentrifuge
Reusable—use prepared peptide resin several times with no significant loss of capacity

The AminoLink Plus Micro Immobilization Kit is for immobilizing small amounts (25 to 100 μg) of purified antibody and other proteins onto Thermo Scientific AminoLink Plus Coupling Resin (crosslinked 4% beaded agarose) to create a small, reusable, microcentrifuge affinity column. The coupling and affinity purification procedures are optimized for small sample volumes (200 to 300 μL). Wash and elution steps are achieved rapidly and efficiently with the convenient microcentrifuge spin columns. Each kit contains sufficient reagents for 10 coupling reactions and 20 affinity purifications.

Related Products
AminoLink™ Plus Coupling Resin
AminoLink™ Plus Immobilization Kit, 2 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
产品规格试剂盒、微量离心机、离心柱
配方多组分试剂盒,含 10 x 0.1 mL 树脂柱
数量10 个色谱柱试剂盒
固定相醛类
足够用于25-100 µg 蛋白/柱
靶标通过伯胺固定抗体、蛋白
容量(公制)固定 25-100 µg 蛋白/柱
产品线AminoLink™
类型固定试剂盒
Unit SizeEach
内容与储存
足够用于:固定 25 至 100 µg 蛋白/柱;包括用于 20 次亲和纯化的缓冲液
•AminoLink Plus 离心柱,0.1 mL,10 个柱
•PBS 包(制成 500 mL),1 包
•氰基硼氢化钠溶液,5 M,0.5 mL
•淬灭缓冲液,60 mL
•洗涤液,25 mL
•洗脱缓冲液,50 mL
•收集管,200 管

在 4°C 下储存。

常见问题解答 (FAQ)

Does sodium azide interfere with AminoLink Support coupling?

No, the gel is stored in sodium azide with no loss in activity. Azide is not a primary amine and is rather unreactive.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I know whether to use pH 7.2 or pH 10 for coupling my protein to AminoLink Plus Gel?

Coupling at pH 10 generally gives some increase in efficiency but may damage some ligands and requires a more involved procedure. If stability of the ligand is in question or the increase in coupling efficiency is of minor importance, use the pH 7.2 coupling procedure. This procedure has fewer steps and will still give good coupling efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink and AminoLink Plus supports?

AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do your AminoLink Coupling Resin and AminoLink Plus Coupling Resin work?

AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.