AminoLink Plus 树脂是经过改良含醛基的微珠状琼脂糖载体。抗体、其他蛋白或具有伯胺的任何分子可以通过优化的还原胺化形式与树脂相结合。一旦固定了一个抗体或其他配体,就可将制备好的亲和树脂用于各种涉及批或离心柱色谱分析的亲和纯化方法。树脂和连结在亲和色谱分析中通常使用的结合和洗脱条件下保持稳定,使得制备好的树脂可用于至少 10 次亲和纯化。
足够用于:固定 2 至 40 mg 蛋白/柱 •AminoLink Plus 柱,2 mL,5 个柱 • PBS 包(制成 500 mL),1 包 • 柠檬酸碳酸盐缓冲包(制成 500 mL),1 包 • 氰基硼氢化钠溶液,5M,0.5 mL • 淬灭缓冲液,60 mL • 洗涤液,240 mL • 柱附件
在 4°C 下储存
常见问题解答 (FAQ)
What is the binding capacity of the AminoLink Coupling Resin or aminoLink Plus Resin?
AminoLink Coupling Resin can bind approximately 10 mg of IgG/mL of resin bed, while AminoLink Plus Resin can bind up to 15-20 mg of IgG/mL of resin bed. The upper limit for binding peptides is approximately 1 mg/mL of resin for both support types.
What are the differences between the two supports for the AminoLink Plus Resin?
The AminoLink Plus Resin uses a type of agarose that has better flow properties, which may shorten the time needed for drip-column methods. It is also activated at a higher level, allowing greater ligand to be immobilized.
My ligand is not soluble in the coupling buffer when using the AminoLink Coupling Resins or Kits. What can I do?
Coupling reactions with AminoLink Resins are compatible with 3-4 M fresh urea or guanidine. (However, old solutions of urea may contain ammonia, which will interfere with ligand immobilization.) Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to coupling buffer so that the DMSO does not exceed 20% of the final solution.
How long will the coupling reaction take when using the AminoLink Resins and Kits?
The immobilizatoin reaction takes six hours; the entire procedure may require seven to eight hours. This time can be decreased if the high pH protocol for AminoLink Plus Resin is used.