EZ-Link™ Sulfo NHS-LC-LC-生物素，No-Weigh™ 规格
EZ-Link™ Sulfo NHS-LC-LC-生物素，No-Weigh™ 规格
引用和文献 (14)
Thermo Scientific™

EZ-Link™ Sulfo NHS-LC-LC-生物素，No-Weigh™ 规格

Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-生物素可对抗体、蛋白和任何其他含伯胺基大分子进行简单、高效的生物素标记。对于这些独特的水溶性、膜不渗透性试剂，细胞表面蛋白的特异性标记是另一种常见应用。Thermo Scientific了解更多信息
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货号数量
2133850 mg
A3535810 x 1 mg
货号 21338
价格（CNY)
4,552.00
Each
添加至购物车
数量:
50 mg
请求批量或定制报价
价格（CNY)
4,552.00
Each
添加至购物车
Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-生物素可对抗体、蛋白和任何其他含伯胺基大分子进行简单、高效的生物素标记。对于这些独特的水溶性、膜不渗透性试剂，细胞表面蛋白的特异性标记是另一种常见应用。

Thermo Scientific No-Weigh 产品是可提供预分装规格的特殊试剂。预称重的包装可避免重复开瓶和关盖，从而防止随时间推移导致的试剂反应性的降低和污染。对于这种规格，可每次使用一小瓶新鲜试剂，避免了对少量试剂进行称重的麻烦，并减少了对试剂稳定性的担忧。

EZ-Link Sulfo-NHS-LC-LC-生物素的特点：

•蛋白标记—生物素化抗体有助于固定、纯化或检测（使用链霉素亲和素树脂或探针）
• 细胞表面标记—仅对全细胞表面蛋白进行生物素化，因为带负电荷的试剂不会渗透细胞膜
• 胺反应性—与伯胺 (-NH2)（如赖氨酸 (K) 侧链或多肽氨基端）反应
• 可溶性—与普通 NHS 酯化合物相比带电荷的 sulfo-NHS 基团可提高试剂级水溶性
• 不可逆—形成永久酰胺键；间隔臂不可裂解
• 双倍长度—间隔臂（添加至靶标的总长度）为 30.5 埃，该扩展臂有助于尽可能减少生物素结合的空间位阻

Sulfo-NHS-LC-LC-生物素是三种非常相似的 EZ-Link 试剂中最长的一种，该试剂具有水溶性，不可裂解，且在溶液中可实现抗体、蛋白和任何其他含伯胺大分子的简单高效生物素化。对于这些独特的水溶性、膜不渗透性试剂，细胞表面蛋白的特异性标记是另一种常见应用。仅在间隔臂长度方面不同的三种 Sulfo-NHS-酯试剂可优化标记和检测实验，且实验中生物素结合的空间位阻是重要因素。

我们生产生物素试剂，以确保尽可能使预期研究应用的总体产品完整性、一致性和性能达到较高水平。

生物素 N-羟基磺基琥珀酰亚胺 (NHS) 酯是较常用的生物素化试剂类型。NHS-活化生物素在碱性缓冲液中与伯胺基团 (-NH2) 高效反应，形成稳定的酰胺键。蛋白（如抗体）通常有几种可作为标记靶标的伯胺，包括赖氨酸 (K) 残基的侧链和每个多肽的 N-末端。

生物素各种 NHS 酯试剂在长度、溶解度、细胞通透性和可裂解性方面有所不同。非磺化 NHS-生物素具有细胞渗透性，但必须在有机溶剂（如 DMSO 或 DMF）中溶解。Sulfo-NHS 生物素（和带有聚乙二醇化间隔的生物素）可以直接溶解在水中，但无膜渗透性。含二硫键的各种类型可使用还原剂进行裂解，从而使生物素基团与标记蛋白分离。
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性细胞不可透过性
标签类型生物素& 类似物
产品线EZ-Link
产品类型Sulfo-NHS-LC-LC-生物素
数量50 mg
反应一部分活性酯、琥珀酰亚胺酯、Sulfo-NHS 酯
化学反应性胺
标签或染料生物素
溶解度DMF（二甲基甲酰胺）, DMSO（二甲亚砜）, 水
间隔子长
Unit SizeEach
内容与储存
在 -20°C 下干燥储存。在环境温度下运输。

常见问题解答 (FAQ)

Can you provide the shelf-life for EZ-Link Sulfo-NHS-LC-LC-Biotin?

EZ-Link Sulfo-NHS-LC-LC-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between EZ-Link Sulfo-NHS-LC-Biotin and Sulfo-NHS-LC-LC-Biotin?

EZ-Link Sulfo-NHS-LC-Biotin has a spacer arm length of 22.4 Angstroms while EZ-Link Sulfo-NHS-LC-LC-Biotin has a spacer arm length of 30.5 Angstroms.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is there any advantage of choosing a Sulfo-NHS biotin with a longer spacer arm than EZ-Link Sulfo NHS Biotin?

Sometimes. The biotin has to insert 9 angstrom into the pocket of avidin, streptavidin or NeutrAvidin to bind. If steric hindrance may be a problem, using a biotinylation reagent with a longer spacer arm (Sulfo-NHS-LC Biotin or Sulfo-NHS-LC-LC Biotin) may result in greater purification or deteciton.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (14)

引用和文献
Abstract
Systematic and standardized comparison of reported amyloid-ß receptors for sufficiency, affinity, and Alzheimer's disease relevance.
Authors:Smith LM, Kostylev MA, Lee S, Strittmatter SM
Journal:J Biol Chem
PubMed ID:30787106
'Oligomeric assemblies of amyloid-ß (Aß) peptide (Aßo) in the brains of individuals with Alzheimer''s disease (AD) are toxic to neuronal synapses. More than a dozen Aß receptor candidates have been suggested to be responsible for various aspects of the molecular pathology and memory impairment in mouse models of AD. A ... More
TMEM87a/Elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration.
Authors:Patkunarajah A, Stear JH, Moroni M, Schroeter L, Blaszkiewicz J, Tearle JL, Cox CD, Fürst C, Sánchez-Carranza O, Ocaña Fernández MDÁ, Fleischer R, Eravci M, Weise C, Martinac B, Biro M, Lewin GR, Poole K
Journal:Elife
PubMed ID:32228863
'Mechanoelectrical transduction is a cellular signalling pathway where physical stimuli are converted into electro-chemical signals by mechanically activated ion channels. We describe here the presence of mechanically activated currents in melanoma cells that are dependent on TMEM87a, which we have renamed Elkin1. Heterologous expression of this protein in PIEZO1-deficient cells, ... More
Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system.
Authors:Wu Z, Harne R, Chintoan-Uta C, Hu TJ, Wallace R, MacCallum A, Stevens MP, Kaiser P, Balic A, Hume DA
Journal:Dev Comp Immunol
PubMed ID:31870792
'Macrophage colony-stimulating factor (CSF1) is an essential growth factor to control the proliferation, differentiation and survival of cells of the macrophage lineage in vertebrates. We have previously produced a recombinant chicken CSF1-Fc fusion protein and administrated it to birds which produced a substantial expansion of tissue macrophage populations. To further ... More
Different SUMO paralogues determine the fate of wild-type and mutant CFTRs: biogenesis versus degradation.
Authors:Gong X, Liao Y, Ahner A, Larsen MB, Wang X, Bertrand CA, Frizzell RA
Journal:Mol Biol Cell
PubMed ID:30403549
'A pathway for cystic fibrosis transmembrane conductance regulator (CFTR) degradation is initiated by Hsp27, which cooperates with Ubc9 and binds to the common F508del mutant to modify it with SUMO-2/3. These SUMO paralogues form polychains, which are recognized by the ubiquitin ligase, RNF4, for proteosomal degradation. Here, protein array analysis ... More
Studies towards hcTnI Immunodetection Using Electrochemical Approaches Based on Magnetic Microbeads.
Authors:Hernández-Albors A, Colom G, Salvador JP, Marco MP
Journal:Sensors (Basel)
PubMed ID:30060625
'Different electrochemical strategies based on the use of magnetic beads are described in this work for the detection of human cardiac troponin I (hcTnI). hcTnI is also known as the gold standard for acute myocardial infarction (AMI) diagnosis according to the different guidelines from the European Society of Cardiology (ESC) ... More
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