'Hybridoma methods for monoclonal antibody (mAb) cloning are a mainstay of biomedical research, but they are hindered by the need to maintain hybridomas in oligoclonal pools during antibody screening. Here, we describe a system in which hybridomas specifically capture and display the mAbs they secrete: On-Cell mAb Screening (OCMS™). In ... More
HIV-1 Envelope Recognition by Polyreactive and Cross-Reactive Intestinal B Cells.
AuthorsPlanchais C, Kök A, Kanyavuz A, Lorin V, Bruel T, Guivel-Benhassine F, Rollenske T, Prigent J, Hieu T, Prazuck T, Lefrou L, Wardemann H, Schwartz O, Dimitrov JD, Hocqueloux L, Mouquet H
JournalCell Rep
PubMed ID30970259
'Mucosal immune responses to HIV-1 involve the recognition of the viral envelope glycoprotein (gp)160 by tissue-resident B cells and subsequent secretion of antibodies. To characterize the B cells "sensing" HIV-1 in the gut of infected individuals, we probed monoclonal antibodies produced from single intestinal B cells binding to recombinant gp140 ... More
Optimizing ultrasound molecular imaging of secreted frizzled related protein 2 expression in angiosarcoma.
AuthorsTsuruta JK, Schaub NP, Rojas JD, Streeter J, Klauber-DeMore N, Dayton P
JournalPLoS One
PubMed ID28333964
Secreted frizzled related protein 2 (SFRP2) is a tumor endothelial marker expressed in angiosarcoma. Previously, we showed ultrasound molecular imaging with SFRP2-targeted contrast increased average video pixel intensity (VI) of angiosarcoma vessels by 2.2 ± 0.6 VI versus streptavidin contrast. We hypothesized that redesigning our contrast agents would increase imaging ... More
BDNF inhibits neurodegenerative disease-associated asparaginyl endopeptidase activity via phosphorylation by AKT.
AuthorsWang ZH, Wu W, Kang SS, Liu X, Wu Z, Peng J, Yu SP, Manfredsson FP, Sandoval IM, Liu X, Wang JZ, Ye K
JournalJCI Insight
PubMed ID30135302
AEP is an age-dependent lysosomal asparaginyl endopeptidase that cleaves numerous substrates including tau and a-synuclein and mediates their pathological roles in neurodegenerative diseases. However, the molecular mechanism regulating this critical protease remains incompletely understood. Here, we show that Akt phosphorylates AEP on residue T322 upon brain-derived neurotrophic factor (BDNF) treatment ... More
Targeting CD46 for both adenocarcinoma and neuroendocrine prostate cancer.
AuthorsSu Y, Liu Y, Behrens CR, Bidlingmaier S, Lee NK, Aggarwal R, Sherbenou DW, Burlingame AL, Hann BC, Simko JP, Premasekharan G, Paris PL, Shuman MA, Seo Y, Small EJ, Liu B
JournalJCI Insight
PubMed ID30185663
Although initially responsive to androgen signaling inhibitors (ASIs), metastatic castration-resistant prostate cancer (mCRPC) inevitably develops and is incurable. In addition to adenocarcinoma (adeno), neuroendocrine prostate cancer (NEPC) emerges to confer ASI resistance. We have previously combined laser capture microdissection and phage antibody display library selection on human cancer specimens and ... More
Aggregated NETs Sequester and Detoxify Extracellular Histones.
AuthorsKnopf J, Leppkes M, Schett G, Herrmann M, Muñoz LE
JournalFront Immunol
PubMed ID31572386
In response to various infectious and sterile stimuli neutrophils release chromatin decorated with bactericidal proteins, referred to as NETs. Their scaffolds are formed from chromatin fibers which display an apparent diameter of 15-17 nm and mainly consist from DNA (2 nm) and DNA-associated histones (11 nm). The NET-forming strands are ... More
Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration.
AuthorsHwang S, Shin DM, Hong JH
JournalMolecules
PubMed ID31546841
Disulfiram has been used in the treatment of alcoholism and exhibits an anti-tumor effect. However, the intracellular mechanism of anti-tumor activity of Disulfiram remains unclear. In this study, we focused on the modulatory role of Disulfiram via oncogenic factor carbonic anhydrase CA12 and its associated transporter anion exchanger AE2 in ... More
AuthorsHu W, Zhang Y, Sun X, Zhang T, Xu L, Xie H, Li Z, Liu W, Lou J, Chen W
JournalElife
PubMed ID31343409
Fc?RIIB binding to its ligand suppresses immune cell activation. A single-nucleotide polymorphic (SNP) change, I232T, in the transmembrane (TM) domain of Fc?RIIB loses its suppressive function, which is clinically associated with systemic lupus erythematosus (SLE). Previously, we reported that I232T tilts Fc?RIIB's TM domain. In this study, combining with molecular ... More
PSD-95 binding dynamically regulates NLGN1 trafficking and function.
AuthorsJeong J, Pandey S, Li Y, Badger JD, Lu W, Roche KW
JournalProc Natl Acad Sci U S A
PubMed ID31138690
PSD-95 is a scaffolding protein that regulates the synaptic localization of many receptors, channels, and signaling proteins. The NLGN gene family encodes single-pass transmembrane postsynaptic cell adhesion molecules that are important for synapse assembly and function. At excitatory synapses, NLGN1 mediates transsynaptic binding with neurexin, a presynaptic cell adhesion molecule, ... More
Tweety-homolog (
AuthorsHan YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ
JournalExp Neurobiol
PubMed ID31138989
In the brain, a reduction in extracellular osmolality causes water-influx and swelling, which subsequently triggers Cl
Autoantibodies Against ß
AuthorsDu Y, Zhang S, Yu H, Wu Y, Cao N, Wang W, Xu W, Li Y, Liu H
JournalJ Am Heart Assoc
PubMed ID30764693
Background Autoantibodies against the second extracellular loop of the ß
Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process.
AuthorsSarker A, Rathore AS, Gupta RD
JournalMicrob Cell Fact
PubMed ID30642336
The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bioactive protein from such insoluble IBs by solubilization and refolding processes. ... More
Butyrophilin-2A1 Directly Binds Germline-Encoded Regions of the V?9Vd2 TCR and Is Essential for Phosphoantigen Sensing.
AuthorsKarunakaran MM, Willcox CR, Salim M, Paletta D, Fichtner AS, Noll A, Starick L, Nöhren A, Begley CR, Berwick KA, Chaleil RAG, Pitard V, Déchanet-Merville J, Bates PA, Kimmel B, Knowles TJ, Kunzmann V, Walter L, Jeeves M, Mohammed F, Willcox BE, Herrmann T
JournalImmunity
PubMed ID32155411
V?9Vd2 T cells respond in a TCR-dependent fashion to both microbial and host-derived pyrophosphate compounds (phosphoantigens, or P-Ag). Butyrophilin-3A1 (BTN3A1), a protein structurally related to the B7 family of costimulatory molecules, is necessary but insufficient for this process. We performed radiation hybrid screens to uncover direct TCR ligands and cofactors that potentiate ... More