Role of TRIM5a RING domain E3 ubiquitin ligase activity in capsid disassembly, reverse transcription blockade, and restriction of simian immunodeficiency virus.
AuthorsKim J, Tipper C, Sodroski J
JournalJ Virol
PubMed ID21680520
'The mammalian tripartite motif protein, TRIM5a, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5a protein and retrovirus, complete disruption of the TRIM5a RING domain decreases virus-restricting activity to various degrees. TRIM5a exhibits RING domain-dependent E3 ubiquitin ligase activity, but the specific role of ... More
A novel C-terminal mutation resulting in constitutive activation of the Listeria monocytogenes central virulence regulatory factor PrfA.
AuthorsXayarath B, Smart JI, Mueller KJ, Freitag NE
JournalMicrobiology
PubMed ID21835879
'The environmental bacterium Listeria monocytogenes survives and replicates in a variety of diverse ecological niches that range from the soil to the cytosol of infected mammalian cells. The ability of L. monocytogenes to replicate within an infected host requires the expression of a number of secreted bacterial gene products whose ... More
Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2.
AuthorsDeiss K, Kisker C, Lohse MJ, Lorenz K
JournalJ Biol Chem
PubMed ID22610096
'Proteins controlling cellular networks have evolved distinct mechanisms to ensure specificity in protein-protein interactions. Raf kinase inhibitor protein (RKIP) is a multifaceted kinase modulator, but it is not well understood how this small protein (21 kDa) can coordinate its diverse signaling functions. Raf1 and G protein-coupled receptor kinase (GRK) 2 ... More
Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling.
AuthorsSéverin S, Pollitt AY, Navarro-Nuñez L, Nash CA, Mourão-Sá D, Eble JA, Senis YA, Watson SP
JournalJ Biol Chem
PubMed ID21098033
The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). In this study, we show that phosphorylation of CLEC-2 by the snake toxin rhodocytin is abolished in the absence of the tyrosine kinase Syk but is not altered ... More
Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces.
AuthorsMoore B, Miles AJ, Guerra-Giraldez C, Simpson P, Iwata M, Wallace BA, Matthews SJ, Smith DF, Brown KA
JournalJ Biol Chem
PubMed ID21106528
The 57-residue small hydrophilic endoplasmic reticulum-associated protein (SHERP) shows highly specific, stage-regulated expression in the non-replicative vector-transmitted stages of the kinetoplastid parasite, Leishmania major, the causative agent of human cutaneous leishmaniasis. Previous studies have demonstrated that SHERP localizes as a peripheral membrane protein on the cytosolic face of the endoplasmic ... More
Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter.
AuthorsOzvegy-Laczka C, Laczkó R, Hegedus C, Litman T, Várady G, Goda K, Hegedus T, Dokholyan NV, Sorrentino BP, Váradi A, Sarkadi B
JournalJ Biol Chem
PubMed ID18644784
Human ABCG2 is a plasma membrane glycoprotein working as a homodimer or homo-oligomer. The protein plays an important role in the protection/detoxification of various tissues and may also be responsible for the multidrug-resistant phenotype of cancer cells. In our previous study we found that the 5D3 monoclonal antibody shows a ... More
NMR analysis of the structure, dynamics, and unique oligomerization properties of the chemokine CCL27.
AuthorsJansma AL, Kirkpatrick JP, Hsu AR, Handel TM, Nietlispach D
JournalJ Biol Chem
PubMed ID20200157
Chemokines have two essential interactions in vivo, with G protein-coupled receptors, which activate intracellular signaling pathways, and with glycosaminoglycans (GAGs), which are involved in cell surface localization and transport. Although it has been shown that chemokines bind and activate their respective G protein-coupled receptors as monomers, many chemokines oligomerize upon ... More