HIV envelope-mediated, CCR5/a4ß7-dependent killing of CD4-negative ?d T cells which are lost during progression to AIDS.
AuthorsLi H, Pauza CD
JournalBlood
PubMed ID21926353
'HIV infects and replicates in CD4+ T cells but effects on host immunity and disease also involve depletion, hyper-activation, and modification of CD4-negative cell populations. In particular, the depletion of CD4-negative ?d T cells is common to all HIV+ individuals. We found that soluble or cell-associated envelope glycoproteins from CCR5-tropic ... More
Adaptor autoregulation promotes coordinated binding within clathrin coats.
AuthorsHung CW, Aoh QL, Joglekar AP, Payne GS, Duncan MC
JournalJ Biol Chem
PubMed ID22457357
Membrane traffic is an essential process that allows protein and lipid exchange between the endocytic, lysosomal, and secretory compartments. Clathrin-mediated traffic between the trans-Golgi network and endosomes mediates responses to the environment through the sorting of biosynthetic and endocytic protein cargo. Traffic through this pathway is initiated by the controlled ... More
A nucleotide phosphatase activity in the nucleotide binding domain of an orphan resistance protein from rice.
Plant resistance proteins (R-proteins) are key components of the plant immune system activated in response to a plethora of different pathogens. R-proteins are P-loop NTPase superfamily members, and current models describe their main function as ATPases in defense signaling pathways. Here we show that a subset of R-proteins have evolved ... More
Surface-affinity profiling to identify host-pathogen interactions.
AuthorsBoleij A, Laarakkers CM, Gloerich J, Swinkels DW, Tjalsma H
JournalInfect Immun
PubMed ID21947776
Proteolytic treatment of intact bacterial cells has proven to be a convenient approach for the identification of surface-exposed proteins. This class of proteins directly interacts with the outside world, for instance, during adherence to human epithelial cells. Here, we aimed to identify host receptor proteins by introducing a preincubation step ... More
Psb27, a transiently associated protein, binds to the chlorophyll binding protein CP43 in photosystem II assembly intermediates.
Photosystem II (PSII), a large multisubunit pigment-protein complex localized in the thylakoid membrane of cyanobacteria and chloroplasts, mediates light-driven evolution of oxygen from water. Recently, a high-resolution X-ray structure of the mature PSII complex has become available. Two PSII polypeptides, D1 and CP43, provide many of the ligands to an ... More