AuthorsTang K, Fu DJ, Julien D, Braun A, Cantor CR, Köster H
JournalProc Natl Acad Sci U S A
PubMed ID10468554
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip ... More
Covalent attachment of synthetic DNA to self-assembled monolayer films.
AuthorsChrisey LA, Lee GU, O'Ferrall CE
JournalNucleic Acids Res
PubMed ID8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Gbeta subunit interacts with a peptide encoding region 956-982 of adenylyl cyclase 2. Cross-linking of the peptide to free Gbetagamma but not the heterotrimer.
AuthorsWeng G, Li J, Dingus J, Hildebrandt JD, Weinstein H, Iyengar R
JournalJ Biol Chem
PubMed ID8900107
The region encoded by amino acids 956-982 of adenylyl cyclase 2 is important for Gbetagamma stimulation. Interactions of a peptide encoding the 956-982 region of adenylyl cyclase 2 (QEHAQEPERQYMHIGTMVEFAYALVGK (QEHA peptide)) with Gbetagamma subunits were studied. QEHA peptide was covalently attached to beta subunit of free Gbetagamma by the cross-linker ... More
Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex.
AuthorsStan T, Brix J, Schneider-Mergener J, Pfanner N, Neupert W, Rapaport D
JournalMol Cell Biol
PubMed ID12640110
BCS1, a component of the inner membrane of mitochondria, belongs to the group of proteins with internal, noncleavable import signals. Import and intramitochondrial sorting of BCS1 are encoded in the N-terminal 126 amino acid residues. Three sequence elements were identified in this region, namely, the transmembrane domain (amino acid residues ... More
Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.
AuthorsAndreadis JD, Chrisey LA
JournalNucleic Acids Res
PubMed ID10606673
We have developed a novel biochemical method to simultaneously amplify and immobilize a target gene onto insoluble particles using PCR. This method employs the covalent attachment of one of two PCR primers to a particle surface either directly during DNA synthesis of the primer or post-DNA synthesis, through the use ... More