DyLight Dyes, rhodamine and fluorescein have been removed successfully using this resin. Samples with dyes in the green spectrum, such as Dylight 488 Dyes, might require double processing because they require a high molar excesses for labeling. The ratio of resin to protein/dye mixture is typically 1:1. When using fluorescent dyes other than those indicated, the ratio of resin to protein/dye mixture will require optimization.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.