Pierce™ 染料去除柱
Pierce™ 染料去除柱
Thermo Scientific™

Pierce™ 染料去除柱

Thermo Scientific Pierce 染料去除柱可有效地结合蛋白溶液中的未结合荧光染料分子,在标记反应后快速纯化荧光偶联抗体和其他蛋白。这些荧光染料去除柱可快速、高效地从蛋白标记反应中去除未反应的荧光染料。标记反应后去除过量的染料通常较为困难且耗时,但对于精确测定染料-蛋白比至关重要。此试剂盒中的染料去除树脂高度专门用于特殊蛋白回收,同时有效去除未结合染料。使用适量的树脂和缓冲液条件,几乎所有的荧光染料均可用这些试剂盒去除。染料去除柱的特点:•快速—在不到一分钟内从标记蛋白溶液中去除游离了解更多信息
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货号 22858
价格(CNY)
3,051.00
Each
添加至购物车
价格(CNY)
3,051.00
Each
添加至购物车
Thermo Scientific Pierce 染料去除柱可有效地结合蛋白溶液中的未结合荧光染料分子,在标记反应后快速纯化荧光偶联抗体和其他蛋白。

这些荧光染料去除柱可快速、高效地从蛋白标记反应中去除未反应的荧光染料。标记反应后去除过量的染料通常较为困难且耗时,但对于精确测定染料-蛋白比至关重要。此试剂盒中的染料去除树脂高度专门用于特殊蛋白回收,同时有效去除未结合染料。使用适量的树脂和缓冲液条件,几乎所有的荧光染料均可用这些试剂盒去除。

染料去除柱的特点:

快速—在不到一分钟内从标记蛋白溶液中去除游离、未结合染料
特异性—纯化树脂提供出色的偶联物回收(75 至 95%)
可定制—单独的树脂和微量离心机柱可优化不同染料和样品浓度
样品友好—处理结果使样品稀释度最小

该产品已成功用于在标记反应后从抗体中去除过量、未结合荧光素、罗丹明和 DyLight 染料。绿色光谱中含染料的样品(如 Dylight 549 和罗丹明)可能需要进行双处理,因为它们需要进行高摩尔过量标记。当使用产品说明中所述荧光染料以外的其他荧光染料时,树脂与蛋白/染料混合物的比值需要进行优化。
For Research Use Only. Not for use in diagnostic procedures.
规格
产品规格试剂盒、微量离心机、离心柱
产品类型染料去除柱
数量5 mL
靶标荧光染料去除
产品线Pierce™
Unit SizeEach

常见问题解答 (FAQ)

Can the Dye Removal Columns be reused?

No. The resin is effective for one-time use only and cannot be regenerated.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Dye Removal resin solvent-compatible?

Yes, the resin is compatible with up to 10% organic solvent such as DMF.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is there sodium azide in the Dye Removal resin's storage solution?

Yes. It contains 0.02% sodium azide.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Which fluorescent dyes can I remove with the Dye Removal resin?

DyLight Dyes, rhodamine and fluorescein have been removed successfully using this resin. Samples with dyes in the green spectrum, such as Dylight 488 Dyes, might require double processing because they require a high molar excesses for labeling. The ratio of resin to protein/dye mixture is typically 1:1. When using fluorescent dyes other than those indicated, the ratio of resin to protein/dye mixture will require optimization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many samples can I process with the Dye Removal Columns?

The kit includes sufficient material to process 10 samples of 250 µl each for most dyes. The kit contains 5 mL of Dye Removal Resin, 10 Spin Columns and 20 Collection Tubes. The ideal labeled-protein concentration range is 1-2.5 mg/mL.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.