Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma.
AuthorsZekry A
JournalNature communications
PubMed ID33420074
Measurement of protein using bicinchoninic acid.
AuthorsSmith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC
JournalAnal Biochem
PubMed ID3843705
Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The ... More
Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo.
AuthorsMartin I, Cabán-Hernández K, Figueroa-Santiago O, Espino AM
Journal
PubMed ID25780044
TLR4, the innate immunity receptor for bacterial endotoxins, plays a pivotal role in the induction of inflammatory responses. There is a need to develop molecules that block either activation through TLR4 or the downstream signaling pathways to inhibit the storm of inflammation typically elicited by bacterial LPS, which is a ... More
Human non-small cell lung tumors and cells derived from normal lung express both estrogen receptor alpha and beta and show biological responses to estrogen.
AuthorsStabile LP, Davis AL, Gubish CT, Hopkins TM, Luketich JD, Christie N, Finkelstein S, Siegfried JM
JournalCancer Res
PubMed ID11929836
Lung cancer is becoming increasingly common in women and in the United States accounts for more female cancer deaths annually than breast cancer. Many epidemiological studies have provided evidence that women are more susceptible than men to the adverse effects of tobacco smoke. These observations suggest the possible role of ... More
Food Perception Primes Hepatic ER Homeostasis via Melanocortin-Dependent Control of mTOR Activation.
AuthorsBrandt C, Nolte H, Henschke S, Engström Ruud L, Awazawa M, Morgan DA, Gabel P, Sprenger HG, Hess ME, Günther S, Langer T, Rahmouni K, Fenselau H, Krüger M, Brüning JC
JournalCell
PubMed ID30445039
'Adaptation of liver to the postprandial state requires coordinated regulation of protein synthesis and folding aligned with changes in lipid metabolism. Here we demonstrate that sensory food perception is sufficient to elicit early activation of hepatic mTOR signaling, Xbp1 splicing, increased expression of ER-stress genes, and phosphatidylcholine synthesis, which translate ... More
Continual conscious bioluminescent imaging in freely moving somatotransgenic mice.
AuthorsKarda R, Perocheau DP, Suff N, Ng J, Delhove JMKM, Buckley SMK, Richards S, Counsell JR, Hagberg H, Johnson MR, McKay TR, Waddington SN
JournalSci Rep
PubMed ID28743959
'Luciferase bioimaging in living animals is increasingly being applied in many fields of biomedical research. Rodent imaging usually involves anaesthetising the animal during data capture, however, the biological consequences of anaesthesia have been largely overlooked. We have evaluated luciferase bioimaging in conscious, unrestrained mice after neonatal intracranial or intravascular administration ... More
Proteomic Screen for Cellular Targets of the Vaccinia Virus F10 Protein Kinase Reveals that Phosphorylation of mDia Regulates Stress Fiber Formation.
AuthorsGreseth MD, Carter DC, Terhune SS, Traktman P
JournalMol Cell Proteomics
PubMed ID28183815
'Vaccinia virus, a complex dsDNA virus, is unusual in replicating exclusively within the cytoplasm of infected cells. Although this prototypic poxvirus encodes >200 proteins utilized during infection, a significant role for host proteins and cellular architecture is increasingly evident. The viral B1 kinase and H1 phosphatase are known to target ... More
Tissue factor-specific ultra-bright SERRS nanostars for Raman detection of pulmonary micrometastases.
AuthorsNayak TR, Andreou C, Oseledchyk A, Marcus WD, Wong HC, Massagué J, Kircher MF
JournalNanoscale
PubMed ID27991632
'Here we demonstrate a novel application of ''surface enhanced resonance Raman scattering nanoparticles'' (SERRS NPs) for imaging breast cancer lung metastases with much higher precision than currently feasible. A breast cancer lung metastasis mouse model was established by intravenous injection of LM2 cells. These mice were intravenously administered SERRS NPs ... More
Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis.
AuthorsKind M, Klukowska-Rötzler J, Berezowska S, Arcaro A, Charles RP
JournalPLoS One
PubMed ID29088297
'PI3K signaling is frequently dysregulated in NSCLC-SQCC. In contrast to well characterized components of the PI3K signaling network contributing to the formation of SQCC, potential oncogenic effects of alterations in PIK3C2B are poorly understood. Here, a large cohort (n = 362) of NSCLC-SQCC was selectively screened for four reported somatic ... More
HPV-Mediated Resistance to TNF and TRAIL Is Characterized by Global Alterations in Apoptosis Regulatory Factors, Dysregulation of Death Receptors, and Induction of ROS/RNS.
AuthorsCabeça TK, de Mello Abreu A, Andrette R, de Souza Lino V, Morale MG, Aguayo F, Termini L, Villa LL, Lepique AP, Boccardo E
JournalInt J Mol Sci
PubMed ID30625987
'Persistent infection with high-risk human papilloma virus (HR-HPV) is the main risk factor for the development of invasive cervical cancer although is not sufficient to cause cervical cancer. Several host and environmental factors play a key role in cancer initiation/progression, including cytokines and other immune-response mediators. Here, we characterized the ... More
A gut microbial factor modulates locomotor behaviour in Drosophila.
AuthorsSchretter CE, Vielmetter J, Bartos I, Marka Z, Marka S, Argade S, Mazmanian SK
JournalNature
PubMed ID30356215
While research into the biology of animal behaviour has primarily focused on the central nervous system, cues from peripheral tissues and the environment have been implicated in brain development and function ... More
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo.
AuthorsIbraheim R, Song CQ, Mir A, Amrani N, Xue W, Sontheimer EJ
JournalGenome Biol
PubMed ID30231914
Clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) have recently opened a new avenue for gene therapy. Cas9 nuclease guided by a single-guide RNA (sgRNA) has been extensively used for genome editing. Currently, three Cas9 orthologs have been adapted for in vivo genome engineering applications: Streptococcus pyogenes ... More
Deciphering molecular mechanism of silver by integrated omic approaches enables enhancing its antimicrobial efficacy in E. coli.
AuthorsWang H, Yan A, Liu Z, Yang X, Xu Z, Wang Y, Wang R, Koohi-Moghadam M, Hu L, Xia W, Tang H, Wang Y, Li H, Sun H
JournalPLoS Biol
PubMed ID31181061
Despite the broad-spectrum antimicrobial activities of silver, its internal usage is restricted, owing to the toxicity. Strategies to enhance its efficacy are highly desirable but rely heavily on the understanding of its molecular mechanism of action. However, up to now, no direct silver-targeting proteins have been mined at a proteome-wide ... More
CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release.
AuthorsAjasin DO, Rao VR, Wu X, Ramasamy S, Pujato M, Ruiz AP, Fiser A, Bresnick AR, Kalpana GV, Prasad VR
JournalElife
PubMed ID31172941
Cellular ESCRT machinery plays pivotal role in HIV-1 budding and release. Extracellular stimuli that modulate HIV-1 egress are currently unknown. We found that CCL2 induced by HIV-1 clade B (HIV-1B) infection of macrophages enhanced virus production, while CCL2 immuno-depletion reversed this effect. Additionally, HIV-1 clade C (HIV-1C) was refractory to ... More
Ursolic acid alleviates airway-vessel remodeling and muscle consumption in cigarette smoke-induced emphysema rats.
AuthorsLin L, Hou G, Han D, Yin Y, Kang J, Wang Q
JournalBMC Pulm Med
PubMed ID31170951
This study assessed the effects of ursolic acid (UA) on airway-vessel remodeling and muscle atrophy in cigarette smoke (CS)-induced emphysema rats and investigated potential underlying mechanisms. ... More