Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for theranostic application.
AuthorsMoura CC, Segundo MA, Neves Jd, Reis S, Sarmento B
Journal
PubMed ID25364249
Rheumatoid arthritis (RA) is an autoimmune disease with severe consequences for the quality of life of sufferers. Regrettably, the inflammatory process involved remains unclear, and finding successful therapies as well as new means for its early diagnosis have proved to be daunting tasks. As macrophages are strongly associated with RA ... More
Differences in sperm protein abundance and carbonylation level in bull ejaculates of low and high quality.
AuthorsMostek A, Westfalewicz B, Slowinska M, Dietrich MA, Judycka S, Ciereszko A
JournalPLoS One
PubMed ID30427859
'In breeding and insemination centres, significant variation in bull ejaculate quality is often observed between individuals and also within the same individual. Low-quality semen does not qualify for cryopreservation and is rejected, generating economic loss. The mechanisms underlying the formation of low-quality ejaculates are poorly understood; therefore, the aim of ... More
Mitogen-Activated Protein Kinase Cross-Talk Interaction Modulates the Production of Melanins in Aspergillus fumigatus.
AuthorsManfiolli AO, Siqueira FS, Dos Reis TF, Van Dijck P, Schrevens S, Hoefgen S, Föge M, Straßburger M, de Assis LJ, Heinekamp T, Rocha MC, Janevska S, Brakhage AA, Malavazi I, Goldman GH, Valiante V
JournalMBio
PubMed ID30914505
'The pathogenic fungus'
ICAM3-Fc Outperforms Receptor-Specific Antibodies Targeted Nanoparticles to Dendritic Cells for Cross-Presentation.
AuthorsCruz LJ, Tacken PJ, van der Schoot JMS, Rueda F, Torensma R, Figdor CG
JournalMolecules
PubMed ID31083610
'Optimal targeting of nanoparticles (NP) to dendritic cells (DCs) receptors to deliver cancer-specific antigens is key to the efficient induction of anti-tumour immune responses. Poly (lactic-co-glycolic acid) (PLGA) nanoparticles containing tètanus toxoid and gp100 melanoma-associated antigen, toll-like receptor adjuvants were targeted to the DC-SIGN receptor in DCs by specific humanized ... More
Metabolic shift from glycogen to trehalose promotes lifespan and healthspan in
AuthorsSeo Y, Kingsley S, Walker G, Mondoux MA, Tissenbaum HA
JournalProc Natl Acad Sci U S A
PubMed ID29511104
As Western diets continue to include an ever-increasing amount of sugar, there has been a rise in obesity and type 2 diabetes. To avoid metabolic diseases, the body must maintain proper metabolism, even on a high-sugar diet. In both humans and ... More
Per-Arnt-Sim Kinase (PASK) Deficiency Increases Cellular Respiration on a Standard Diet and Decreases Liver Triglyceride Accumulation on a Western High-Fat High-Sugar Diet.
AuthorsPape JA, Newey CR, Burrell HR, Workman A, Perry K, Bikman BT, Bridgewater LC, Grose JH
JournalNutrients
PubMed ID30558306
Diabetes and the related disease metabolic syndrome are epidemic in the United States, in part due to a shift in diet and decrease in physical exercise. PAS kinase is a sensory protein kinase associated with many of the phenotypes of these diseases, including hepatic triglyceride accumulation and metabolic dysregulation in ... More
The Proanthocyanidin-Rich Fraction Obtained from Red Rice Germ and Bran Extract Induces HepG2 Hepatocellular Carcinoma Cell Apoptosis.
This study aims to determine the anti-carcinogenic effects of the proanthocyanidin-rich fraction (PRFR) obtained from red rice germ and bran extract on HepG2 cells. The PRFR obtained from red rice germ and bran extract could reduce the cell viability of HepG2 cells as shown by the IC ... More
Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery.
AuthorsLavoie RA, di Fazio A, Blackburn RK, Goshe MB, Carbonell RG, Menegatti S
JournalInt J Mol Sci
PubMed ID30965558
The growing integration of quality-by-design (QbD) concepts in biomanufacturing calls for a detailed and quantitative knowledge of the profile of impurities and their impact on the product safety and efficacy. Particularly valuable is the determination of the residual level of host cell proteins (HCPs) secreted, together with the product of ... More
Application of the uridine auxotrophic host and synthetic nucleosides for a rapid selection of hydrolases from metagenomic libraries.
AuthorsUrbeliene N, Kutanovas S, Meškiene R, Gasparaviciute R, Tauraite D, Koplunaite M, Meškys R
JournalMicrob Biotechnol
PubMed ID30302933
A high-throughput method (= 10
Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response.
AuthorsMaidarti M, Clarkson YL, McLaughlin M, Anderson RA, Telfer EE
JournalHum Reprod
PubMed ID30521029
Does ovarian follicle activation by phosphatase homologue of chromosome-10 (PTEN) inhibition affect DNA damage and repair in bovine oocytes and granulosa cells?