Pierce™ Reversible Protein Stain Kit for PVDF Membranes - FAQs

View additional product information for Pierce™ Reversible Protein Stain Kit for PVDF Membranes - FAQs (24585)

11 product FAQs found

我在使用MemCode可逆性蛋白质染色剂试剂盒时，发现染色剂不是完全可逆的。这是为什么？

以下是可能原因和解决方案：

- 在逆转处理前，膜是干燥的：保持膜处于湿润状态。
- 样品中的蛋白含量较高：将膜在Stain Eraser溶液中的孵育时间延长至5分钟。 降低样品中的蛋白浓度。

我使用MemCode可逆性蛋白质染色剂试剂盒时，条带很微弱或完全看不见。这是为什么？

这可能是因为样品中的蛋白质含量很低或无蛋白质。请检测原始样品中的蛋白浓度。

用于硝化纤维素膜的MemCode可逆性蛋白质染色试剂盒的灵敏度是多少？

用于硝化纤维素膜的MemCode可逆性蛋白质染色试剂盒可检测到蛋白质含量低至25-50 ng的条带。

用于硝化纤维素膜的Thermo Scientific MemCode可逆性蛋白质染色试剂盒应如何保存？保质期是多久？

用于硝化纤维素膜的MemCode可逆性蛋白质染色试剂盒可在室温下稳定保存1年。

Are SuperBlock Blocking Buffers compatible with the Pierce Reversible Protein Stain Kit for PVDF Membranes (Cat. No. 24585)?

The procedure for the Reversible Protein Stain Kit for PVDF Membranes (Cat. No. 24585) is performed before the membrane is blocked with the blocking buffer, and thus, has no effect on the subsequent blocking.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How can I increase the signal intensity when I am using SuperSignal West Femto Maximum Sensitivity Substrate?

The following are possible causes for weak signal intensity:
- Low level of protein transferred from the gel to the membrane. This is particularly true for high molecular weight proteins. Reversible Protein Stain Kits for Nitrocellulose and PVDF membranes (Cat. Nos. 24580 and 24585, respectively) may be used to stain the bands on the membrane to indicate transfer efficiency.
- Over-diluted primary and secondary antibody probes. We suggest using the recommended dilution for the antibody probes for each substrate. More sensitive substrates will require a more dilute antibody solution.
- Short substrate incubation time. For best results, incubate the membrane for 5-15 min in the SuperSignal substrate.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the signal intensity when I am using SuperSignal West Dura Extended Duration Substrate?

The following are possible causes for weak signal intensity:
- Low level of protein transferred from the gel to the membrane. This is particularly true for high molecular weight proteins. Reversible Protein Stain Kits for Nitrocellulose and PVDF membranes (Cat. Nos. 24580 and 24585, respectively) may be used to stain the bands on the membrane to indicate transfer efficiency.
- Over-diluted primary and secondary antibody probes. We suggest using the recommended dilution for the antibody probes for each substrate. More sensitive substrates will require a more dilute antibody solution.
- Short substrate incubation time. For best results, incubate the membrane for 5-15 min in the SuperSignal substrate.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the MemCode Reversible Protein Stain Kit but the stain is not completely reversible. Why is this?

Here are possible causes and solutions:

- Membrane was allowed to dry before reversing. Keep the membrane wet.
- High protein concentration in the sample. Extend incubation in the Stain Eraser up to 5 minutes. Reduce the protein concentration in the sample.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the MemCode Reversible Protein Stain Kit and my bands are faint or not visible at all. What is going on?

This is likely due to low amounts or no protein present in the sample. Please determine the protein concentration in the original sample.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of the MemCode Reversible Protein Stain Kit for Nitrocellulose Membranes?

The MemCode Reversible Protein Stain Kit for Nitrocellulose Membranes detects as low as 25 to 50 ng protein per band.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How should I store the Thermo Scientific MemCode Reversible Protein Stain Kit for Nitrocellulose Membranes and what is its shelf life?

We recommend storing the MemCode Reversible Protein Stain Kit for Nitrocellulose Membranes at room temperature where it is stable for a year.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.