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Imperial™ Protein Stain, 1 L - FAQs

Imperial™ Protein Stain, 1 L - FAQs

查看更多产品信息 Imperial™ Protein Stain - FAQs (24615, 24617)

14 个常见问题解答

Imperial蛋白质染色剂的瓶子中有可见的聚集物。这属于正常情况吗？

所有Coomassie蛋白质染色剂试剂都会形成染色剂-染色剂聚集物，Imperial蛋白质染色剂含有可减慢聚集物形成速度的添加剂。如果不加以干涉，试剂会形成可见的聚集物并在瓶中沉淀；但是，轻轻混合可使这些聚集物完全散开。为了确保使用均匀分散的样品，应在倒出或配制试剂前将其轻轻混合。

使用Imperial蛋白质染色剂对蛋白质凝胶染色后，未得到任何条带。这是为什么？

以下是可能原因和解决方案：

- 样品中无蛋白质：使用一个已知含量的纯蛋白质作为对照
- 样品中的蛋白质含量不足：增加总蛋白上样量
- 未将SDS从凝胶上完全去除：开始染色前，更彻底地洗涤凝胶

我在使用Thermo Scientific Imperial蛋白质染色剂对蛋白质凝胶进行染色时，得到了很高的背景。你们有何建议？

这最有可能是因为SDS的干扰。我们建议在开始染色前，彻底洗涤凝胶。

Imperial蛋白质染料是否兼容质谱分析？

兼容，点击这里查看如何处理经Imperial染料染色的凝胶以用于质谱分析。

Imperial蛋白质染料的灵敏度是多少？

使用增强实验方案， Imperial蛋白质染料可在3小时内检测出少于3ng的蛋白条带。

Imperial蛋白质染料是否含有Coomassie G-250或Coomassie R-250？

Imperial蛋白质染料含有Coomassie R-250。其他成分是保密的。

Thermo Scientific Imperial蛋白质染料应如何保存？保质期是多久？

Imperial蛋白质染料推荐室温保存，可在室温下稳定保存1年。

I am seeing visible aggregates in my bottle of Imperial Protein Stain. Is this normal?

Imperial Protein Stain contains additives that help slow formation of dye-dye aggregates, which occur in all Coomassie dye-based protein staining reagents. If left undisturbed, the reagent may form visible aggregates that settle in the bottle; however, gentle mixing completely disperses these aggregates. To ensure that a homogeneous sample of the reagent is used, mix reagent before pouring or dispensing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining my protein gel with Imperial Protein Stain, I am not seeing any band development. What went wrong?

Here are possible causes and solutions:

- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I stained my protein gel with Thermo Scientific Imperial Protein Stain and am getting very high background. Do you have any tips?

This is most likely due to SDS interference. We recommend washing the gel extensively before starting the staining procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the Imperial Protein Stain compatible with mass spectrometry analysis?

Yes, here (https://tools.thermofisher.com/content/sfs/brochures/TR0050-Stained-gels-for-MS.pdf) is the procedure for processing gels stained with Imperial Stain, for mass spectrometry analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of the Imperial Protein Stain?

With the enhanced protocol, the Imperial Protein Stain can detect less than 3 ng protein per band in 3 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does Imperial Protein Stain contain Coomassie G-250 or Coomassie R-250?

Imperial Protein Stain contains Coomassie R-250. The rest of the composition is proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How should I store the Thermo Scientific Imperial Protein Stain and what is its shelf life?

We recommend storing the Imperial Protein Stain at room temperature where it is stable for a year.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.