HisPur™ 钴 Superflow 琼脂糖
HisPur™ 钴 Superflow 琼脂糖
Thermo Scientific™

HisPur™ 钴 Superflow 琼脂糖

Thermo Scientific HisPur 钴 Superflow 琼脂糖是一种带二价钴电荷的四配位基螯合 Superflow 6 支持相,可专门用于对多聚组氨酸标签蛋白进行高纯度大规模纯化。HisPur了解更多信息
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货号数量
2522810 mL
252311 L
25230250 mL
2522950 mL
货号 25228
价格(CNY)
1,456.00
Each
添加至购物车
数量:
10 mL
请求批量或定制报价
价格(CNY)
1,456.00
Each
添加至购物车
Thermo Scientific HisPur 钴 Superflow 琼脂糖是一种带二价钴电荷的四配位基螯合 Superflow 6 支持相,可专门用于对多聚组氨酸标签蛋白进行高纯度大规模纯化。

HisPur 钴 Superflow 琼脂糖的特点:

稳健— 高度交联的 6% 琼脂糖 Superflow 微珠状支持相可耐受高达 1200 cm/小时的线性流速,且由于钴离子上结合了四配位基螯合剂而极少发生浸出
高得率— 在 150 cm/小时线性流速下,每 mL 树脂可结合 20 mg 6xHis-GFP;在某些流速下,结合能力可超过 30 mg/mL 树脂
高纯度— 钴与组氨酸标签的特异性结合可大大提高洗脱组分的纯度,使得非特异性蛋白的污染率低于 10%
兼容— 可在各种化学物质和 pH 值环境中保持活性
高性价比— 本树脂经多次清洗和重复利用后仍保持稳定

HisPur 钴 Superflow 琼脂糖是一种高度交联的耐用树脂,在中等规模至大规模 FPLC 纯化作业所用流速下不会发生压缩。此树脂在各种化学剂和 pH 值环境下均保持稳定,适用于常见的原位清洁流程。与固定化金属亲和色谱 (IMAC) 所使用的镍及其他配体相比,钴对 His 标签蛋白纯化具有更高的特异性。HisPur 钴 Superflow 琼脂糖与蛋白的特异性结合可提高洗脱组分的纯度,从而省去了额外的纯化步骤。

应用:
•多聚组氨酸标签蛋白的大规模 FPLC 纯化

亲和色谱是一种常用的快速简便的重组蛋白纯化方法。在这类纯化方法中,有一种是将多聚组氨酸标签结合到二价金属阳离子上,此金属阳离子则是由固定在磁珠支持相上的螯合剂进行协调。当对 His 标签蛋白进行固定化金属亲和色谱 (IMAC) 纯化时,微珠、螯合剂以及固定金属的类型将会影响这些纯化操作的纯度、得率以及流速性能。

与交联度较低的琼脂糖树脂相比,高度交联的 6% 琼脂糖 Superflow 微珠状支持物可耐受更高的线性流速。本 HisPur 钴 Superflow 琼脂糖实验方案采用温和的洗涤和洗脱条件,通常可获得纯度大于 90% 的目标蛋白。采用 HisPur 钴 Superflow 琼脂糖获得的蛋白纯度通常高于镍 IMAC 树脂,这使得 HisPur 钴 Superflow 琼脂糖成为一步纯化的极佳之选。此外,对于那些要求树脂在中等压力水平下不出现压缩的大规模纯化作业,HisPur 钴 Superflow 琼脂糖是理想之选。

更多产品数据
HisPur 钴 Superflow 琼脂糖的性能表征

相关产品
HisPur™ 钴树脂
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
产品规格瓶装
数量10 mL
固定相金属螯合物、二价钴
靶标His 标签蛋白
形式悬浮液
产品线HisPur™
类型琼脂糖
Unit SizeEach
内容与储存
在 4°C 下储存

常见问题解答 (FAQ)

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between HisPur Nickel Resin and HisPur Cobalt Resin?

Nickel has a higher affinity for histidines than does cobalt. It binds multiple histidines more tightly than cobalt, and requires more stringent conditions than is necessary for cobalt. The lower affinity of cobalt for multiple histidines typically results in less nonspecific binding of histidine-rich proteins that lack a his-tag compared to nickel resins.

HisPur Ni-NTA resin has a high capacity of up to 60 mg of 6xHis-tagged protein per milliliter. It is a versatile resin that can work under both native and denaturing conditions, and can also be used with a variety of lysis reagents and buffer additives.

HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.