Combined effects of the bisphosphonate, zoledronic acid and the aromatase inhibitor letrozole on breast cancer cells in vitro: evidence of synergistic interaction.
AuthorsNeville-Webbe HL, Coleman RE, Holen I,
JournalBr J Cancer
PubMed ID20160726
'Aromatase inhibitors are widely used in the treatment of oestrogen receptor-positive post-menopausal breast cancer. These patients may also be receiving the bisphosphonate, zoledronic acid (ZA) to prevent bone loss or reduce skeletal morbidity in the setting of advanced disease. The potential biological interaction of these two drugs in breast cancer ... More
Isolation, cultivation and characterization of adult murine prostate stem cells.
AuthorsLukacs RU, Goldstein AS, Lawson DA, Cheng D, Witte ON,
JournalNat Protoc
PubMed ID20360765
'The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by ... More
Binding of low affinity N-formyl peptide receptors to G protein. Characterization of a novel inactive receptor intermediate.
Authors Prossnitz E R; Schreiber R E; Bokoch G M; Ye R D;
JournalJ Biol Chem
PubMed ID7738006
G protein-coupled seven-transmembrane-containing receptors, such as the N-formyl peptide receptor (FPR) of neutrophils, likely undergo a conformational change upon binding of ligand, which enables the receptor to transmit a signal to G proteins. We have examined the functional significance of numerous conserved charged amino acid residues proposed to be located ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors Benbow U; Butticè G; Nagase H; Kurkinen M;
JournalJ Biol Chem
PubMed ID8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More