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查看更多产品信息 Pierce™ Co-Immunoprecipitation Kit - FAQs (26149)
5 个常见问题解答
The Pierce Co-Immunoprecipitation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale for more details.
Yes, but the IP Lysis/Wash Buffer is formulated to be an efficient lysing agent that is gentle to proteins. The exclusion of ionic detergents and pH extremes help to preserve protein: protein interactions improving the results of recovering both antigen and its interacting partners at the elution step (versus interating partners being lost in the wash steps).
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The conditioning buffer helps with the wash and especially to provide the correct environment to enhance the efficiency of the low pH Elution Buffer. The overall result is more antigen recoved in the first volume of elution (more concentrated antigen).
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
No, we do not recommend immobilizing an un-purified antibody; a purified antibody is required. The AminoLink Coupling Resin that is included will react with primary amines that are found on the surface of virtually all proteins, resulting in immobilization of many other proteins along with the antibody. This will reduce the amount of antibody immobilized, resulting in less antigen and interacting partners being purified. It can also result in higher background as other proteins in the sample may bind to the other immobilized proteins and co-elute with the antigen and interacting partners, contaminating them.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Yes, the product manual includes “Table 1. Amounts of antibody, coupling resin, wash buffer and elution buffer to use” that allows researchers to scale their affinity purification using 5 - 1,000 µg of antibody. We do not recommend going below 5 µg of antibody. This was the smallest amount of antibody tested inhouse. The rationale to stop at 5 µg of antibody is that it was felt that an extremely strong affinity between the antibody and antigen would be required to get sensitive, reproducible results with less antibody to capture the antigen and interacting partners.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.