Pierce™ 快速抗体分型试剂盒(外加 κ 和 λ)- 小鼠
Pierce™ 快速抗体分型试剂盒(外加 κ 和 λ)- 小鼠
Thermo Scientific™

Pierce™ 快速抗体分型试剂盒(外加 κ 和 λ)- 小鼠

Thermo Scientific Pierce 快速小鼠抗体分型试剂盒(外加 κ 和 λ)是单次使用、基于组织盒的试剂盒,用于小鼠单克隆抗体类别和亚类鉴别的了解更多信息
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货号数量
2617910 次测试
货号 26179
价格(CNY)
6,286.00
Each
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数量:
10 次测试
请求批量或定制报价
价格(CNY)
6,286.00
Each
添加至购物车
Thermo Scientific Pierce 快速小鼠抗体分型试剂盒(外加 κ 和 λ)是单次使用、基于组织盒的试剂盒,用于小鼠单克隆抗体类别和亚类鉴别的 5 分钟侧流试验测定。

该抗体分型试剂盒使用纯化柱,可在向样品孔中移入少量稀释的培养物上清液或腹水液体样品后, 5 分钟内提供单克隆抗体同种型的彩色读数。该试剂盒可测定小鼠 IgG1、IgG2a、IgG2b、IgG3、IgA、IgM 以及轻链鉴别(κ 轻链相对于 λ 轻链)。

Pierce 快速小鼠抗体分型试剂盒的特点:

长期有效—在室温下可稳定至少一年
单步—将稀释的抗体样品添加至组织盒的上样孔中
快速—在 5 分钟内出现表明抗体同种型的条带
灵敏—可检测大于 10 ng/mL 的任何浓度的组织培养基或腹水中的抗体
特异性—该测定不与胎牛血清 (FBS) 发生交叉反应
可靠—结果与基于标准 ELISA 的分型试验相似

仅需将适当稀释的组织培养物上清液或小鼠腹水样品添加至小型组织盒的孔中,即可进行每次 Pierce 快速分型试剂盒试验。卡上内嵌的金色共轭物可与样品中特定类型和亚类的抗体形成水溶性复合物。这些复合物在抗同种型和类型特异性抗体浸渍膜上移动并溶解。结果显示为指示抗体同种型或亚类的红色条带。

确定单克隆抗体的类别和亚类对于计划较佳的免疫球蛋白纯化方法非常有用。例如,小鼠 IgA 和 IgM 最适合按大小纯化(即凝胶排阻)或使用免疫亲和分离柱纯化。小鼠 IgG2a 和 IgG2b 在 pH 值 7-8 下使用固定化蛋白 A 进行纯化,而小鼠 IgG1 在 pH 值 8-9 下与蛋白 A 的结合性最佳。包含 κ 轻链的免疫球蛋白可使用固定化蛋白 L 进行纯化。

Pierce 快速分型试剂盒与组织培养物上清液和小鼠腹水液体兼容,且比传统基于乳胶-微珠的测深尺测定更灵敏,比基于 ELISA 的分型试验快得多。该试剂盒仅需要 0.5 μL 腹水液体、5 至 55 μL 细胞培养物上清液或 1.5 ng 纯化抗体。

相关产品
快速 ELISA 小鼠 mAb 分型试剂盒
Pierce™ 快速抗体分型试剂盒 - 小鼠
仅供科研使用。不可用于诊断程序。
规格
描述Pierce 快速分型试剂盒(外加 κ 和 λ链)- 小鼠
适用于(应用)膜印迹
数量10 次测试
检测同种型测定
检测方法发色
产品线Pierce
靶标特异性靶标特异性
Unit SizeEach
内容与储存
足够用于:10 次同种型测定
• 分型组织盒(每袋三个),10 袋
• 样品稀释液,45 mL

将试剂盒储存在 2–8°C 下。

常见问题解答 (FAQ)

How does the Rapid ELISA Mouse Antibody Isotyping kit (Cat. No. 37503) differ from the Cassette-based Kits (Cat. Nos. 26178 and 26179)?

The ELISA Kit procedure takes approximately 1.5 hours to complete and enables simultaneous testing of multiple samples (12 per plate). The Cassette-based Kits provide results in only 5 minutes but are single-use units that are less practical and more costly per sample than the ELISA Kit. The Cassette-based Kits are less sensitive (minimum antibody concentration is 10 ng/mL) and provide only qualitative assessment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the Pro-Detect assay kits for rapidly isotyping antibodies?

The Pro-Detect Rapid Antibody Isotyping Kit is a lateral-flow assay with high sensitivity for rapid determination of the immunoglobulin class, subclass, and light chain type of monoclonal antibodies. Two different formats of rapid isotyping kits are available. These include dipstick format and cassette-based format. One can use these kits to identify the isotype of a mouse, rat, or human antibody. Make sure to follow the recommended dilution guidelines.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using a Pro-Detect assay kit and cannot differentiate banding pattern between control lysates and lysates that are actually expressing proteins of interest. What is the problem?

1) It is likely that there could be a substance interfering with the assay. Check the compatibility table on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html), under “Detergent and salt compatibility limits”.
2) The expression level of the actual tagged protein is below the detectable range of the assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Using Pro-Detect assay kits, can I detect the protein of interest in a mixture of proteins such as a crude lysate?

Yes, first, make sure that the protein of interest is tagged and that we offer a Pro-Detect assay kit that can detect that tag. Secondly, make sure that you follow the guidelines to detect proteins in crude lysate and if necessary increase or decrease the lysate concentration to bring the protein of interest within the detectable range of the assay.

Note that the guidelines for the recommended detection ranges reflect the concentration of the pure tagged protein in a given sample mixture.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I interpret the gradual disappearance of four lines in the Pro-Detect competition assay kits?

As the concentration of the tag in the protein sample increases, the number of lines will decrease until they all disappear. The concentration of the tagged proteins is inversely related to the number of lines appearing on the strip.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.