ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response.
AuthorsLian H, Wei J, Zang R, Ye W, Yang Q, Zhang XN, Chen YD, Fu YZ, Hu MM, Lei CQ, Luo WW, Li S, Shu HB
JournalNat Commun
PubMed ID30135424
'Cyclic GMP-AMP synthase (cGAS) senses double-strand (ds) DNA in the cytosol and then catalyzes synthesis of the second messenger cGAMP, which activates the adaptor MITA/STING to initiate innate antiviral response. How cGAS activity is regulated remains enigmatic. Here, we identify ZCCHC3, a CCHC-type zinc-finger protein, as a positive regulator of ... More
Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes.
AuthorsRichards SM, Hovhannisyan N, Gilliham M, Ingram J, Skadhauge B, Heiniger H, Llamas B, Mitchell KJ, Meachen J, Fincher GB, Austin JJ, Cooper A
JournalPLoS One
PubMed ID30716066
'Hybridization capture with in-solution oligonucleotide probes has quickly become the preferred method for enriching specific DNA loci from degraded or ancient samples prior to high-throughput sequencing (HTS). Several companies synthesize sets of probes for in-solution hybridization capture, but these commercial reagents are usually expensive. Methods for economical in-house probe synthesis ... More
The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response.
AuthorsYang Y, Wang SY, Huang ZF, Zou HM, Yan BR, Luo WW, Wang YY
JournalCell Res
PubMed ID26823206
'Recognition of viral dsRNA by Toll-like receptor 3 (TLR3) leads to induction of interferons (IFNs) and proinflammatory cytokines, and innate antiviral response. Here we identified the RNA-binding protein Mex3B as a positive regulator of TLR3-mediated signaling by expression cloning screens. Cells from Mex3b(-/-) mice exhibited reduced production of IFN-ß in ... More
A Naturally Occurring Deletion in the Effector Domain of H5N1 Swine Influenza Virus Nonstructural Protein 1 Regulates Viral Fitness and Host Innate Immunity.
AuthorsWang J, Zeng Y, Xu S, Yang J, Wang W, Zhong B, Ge J, Yin L, Bu Z, Shu HB, Chen H, Lei CQ, Zhu Q
JournalJ Virol
PubMed ID29563291
Nonstructural protein 1 (NS1) of influenza A virus regulates innate immune responses via various mechanisms. We previously showed that a naturally occurring deletion (the EALQR motif) in the NS1 effector domain of an H5N1 swine-origin avian influenza virus impairs the inhibition of type I interferon (IFN) in chicken fibroblasts and ... More
Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response.
AuthorsIijima K, Kobayashi J, Ishizaka Y
JournalRetrovirology
PubMed ID29338752
Viral protein R (Vpr) is an accessory protein of HIV-1, which is potentially involved in the infection of macrophages and the induction of the ataxia-telangiectasia and Rad3-related protein (ATR)-mediated DNA damage response (DDR). It was recently proposed that the SLX4 complex of structure-specific endonuclease is involved in Vpr-induced DDR, which ... More