Plus 活化过氧化物酶是辣根过氧化物酶 (HRP),其天然碳水化合物(糖)已经过高碘酸盐轻微氧化,形成了胺反应性醛基。Plus 活化过氧化物酶的这些羰基可自发有效地与抗体或其他蛋白质上的伯胺交联。该方法比其他胺反应性化学试剂的(如戊二醛偶联)偶联效率更高,戊二醛常常会导致聚合作用,偶联物失活程度更高。预活化酶避免了从头开始制备和验证活化过氧化物酶过程中所固有的困难。本试剂盒提供了酶、辅助试剂和方案,易于生成高性能的偶联物,可在 Western 印迹、ELISA 等检测技术中使用。
What would be the best way to crosslink an antibody with horseradish peroxidase (‘HRP’ unmodified)?
A commonly used method for conjugating HRP to antibodies is by activation of the HRP using sodium meta-periodate (Cat no. 20504) to generate aldehydes. After the reaction, the HRP must be dialyzed to remove the meta-periodate; then the aldehydes on the HRP will react directly to the amine groups on the antibody. Alternatively, we offer activated HRP (Cat. No. 31488) as an alternative to avoid the periodate oxidation step to modify the HRP.