RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
AuthorsBuratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE,
JournalMol Cell Biol
PubMed ID14729981
'In humans, inclusion or exclusion of the fibronectin EDA exon is mainly regulated by a polypurinic enhancer element (exonic splicing enhancer [ESE]) and a nearby silencer element (exonic splicing silencer [ESS]). While human and mouse ESEs behave identically, mutations introduced into the homologous mouse ESS sequence result either in no ... More
RNA splicing capability of live neuronal dendrites.
AuthorsGlanzer J, Miyashiro KY, Sul JY, Barrett L, Belt B, Haydon P, Eberwine J,
JournalProc Natl Acad Sci U S A
PubMed ID16275927
Dendrites are specialized extensions of the neuronal soma that contain components of the cellular machinery involved in RNA and protein metabolism. Several dendritically localized proteins are associated with the precursor-mRNA (pre-mRNA) splicing complex, or spliceosome. Although some spliceosome-related, RNA-binding proteins are known to subserve separate cytoplasmic functions when moving between ... More
The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B.
AuthorsAnderson ES, Lin CH, Xiao X, Stoilov P, Burge CB, Black DL
JournalRNA
PubMed ID22456266
Modulation of alternative pre-mRNA splicing is a potential approach to therapeutic targeting for a variety of human diseases. We investigated the mechanism by which digitoxin, a member of the cardiotonic steroid class of drugs, regulates alternative splicing. Transcriptome-wide analysis identified a large set of alternative splicing events that change after ... More