GlutaMAX™ Supplement
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GlutaMAX™ Supplement
Citations & References (4)
Gibco™

GlutaMAX™ Supplement

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GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine. It exhibits increased stability over L-glutamine leading to improved cellular health.
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Catalog NumberQuantity
3505007920 x 100 mL
35050061100 mL
Catalog number 35050079
Price (CNY)
14,102.00
Each
Add to cart
Quantity:
20 x 100 mL
Price (CNY)
14,102.00
Each
Add to cart

Gibco GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine that exhibits increased stability, leading to improved cell health. L-Glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. GlutaMAX Supplement is suitable for adherent and suspension culture of mammalian cells, with no adaptation required. It is formulated as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl.

Compared to L-glutamine, GlutaMAX Supplement:

  • Minimizes toxic ammonia build-up
  • Improves cell viability and growth
  • Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up

Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth

Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures

GlutaMAX Supplement is room-temperature stable and ready to use. It retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.

Specifications
Cell TypeMammalian Cells
Concentration100 X
Culture TypeMammalian Cell Culture
For Use With (Application)Clinical Research, Translational Research
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality440 - 500 mOsm/kg
Product LineGibco, GlutaMAX
Quantity20 x 100 mL
Shelf Life24 Months
SourceAnimal origin-free
ClassificationAnimal Origin-free
FormLiquid
Product TypeGlutaMax
SterilitySterile-filtered
pH4.7 to 6
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Alternate storage conditions: -5°C to -20°C and 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

Why is GlutaMAX Supplement (Cat. Nos. 35050061, 35050079) recommended over L-glutamine when reconstituting Geltrex Reduced Growth Factor Basement Membrane Matrix?

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) require supplementation for use?

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do my cells need to adapt to GlutaMAX Supplement (Cat. No. 35050061, 35050079)?

Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all GlutaMAX™ Supplement FAQs

Citations & References (4)

Citations & References
Abstract
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.
Authors:Kutner RH, Zhang XY, Reiser J,
Journal:Nat Protoc
PubMed ID:19300443
'Over the past decade, lentiviral vectors have emerged as powerful tools for transgene delivery. The use of lentiviral vectors has become commonplace and applications in the fields of neuroscience, hematology, developmental biology, stem cell biology and transgenesis are rapidly emerging. Also, lentiviral vectors are at present being explored in the ... More
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Authors:Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB,
Journal:Nature
PubMed ID:22343901
Recurrent mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 have been identified in gliomas, acute myeloid leukaemias (AML) and chondrosarcomas, and share a novel enzymatic property of producing 2-hydroxyglutarate (2HG) from a-ketoglutarate. Here we report that 2HG-producing IDH mutants can prevent the histone demethylation that is required for lineage-specific progenitor ... More
Characterization of pluripotent stem cells.
Authors:Martí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23306458
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency. Such protocols should ... More