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GlutaMAX™ Supplement, 20 x 100 mL - FAQs

GlutaMAX™ Supplement, 20 x 100 mL - FAQs

查看更多产品信息 GlutaMAX™ Supplement - FAQs (35050061, 35050079)

9 个常见问题解答

清除，缺少或降解谷氨酰胺或生长因子等促生长的基本成份会对培养物的生长速率造成影响么？

是的。如果您怀疑发生了类似情况，请移去培养基并换用新的培养基。或者，您可以添加含有促生长成份的培养基。也可使用I或II型GlutaMAX取代培养基中的谷氨酰胺，以避免谷氨酰胺被耗竭。

配方中含有Gibco GlutaMAX添加剂会改变L-谷氨酰胺的含量么？

在所有以GlutaMAX双肽添加剂作为L-谷氨酰胺替代品的培养基中，谷氨酰胺的浓度都与原始配方中的L-谷氨酰胺浓度处于同等的摩尔水平。

Gibco GlutaMAX与谷氨酰胺添加剂之间的区别是什么？

GlutaMAX-I 添加剂是一款能够改善细胞培养效果的添加剂，是您细胞培养基中L-谷氨酰胺的直接替代品。请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html)来阅读更多关于GlutaMAX添加剂性能的详细信息。

Why is GlutaMAX Supplement (Cat. Nos. 35050061, 35050079) recommended over L-glutamine when reconstituting Geltrex Reduced Growth Factor Basement Membrane Matrix?

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) require supplementation for use?

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do my cells need to adapt to GlutaMAX Supplement (Cat. No. 35050061, 35050079)?

Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Gibco GlutaMAX and glutamine supplements?

GlutaMAX Supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture medium. Please go here to read more about the properties of GlutaMAX supplement (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.