What microplates work best with the SuperSignal ELISA Femto Maximum Sensitivity Substrate?
White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal. Here are two suggestions we offer for white plates:
Can I dilute the SuperSignal ELISA Femto Maximum Sensitivity Substrate working reagent if my signal is too intense?
We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.