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SuperSignal™ ELISA Femto Substrate, 250 mL Kit - FAQs

SuperSignal™ ELISA Femto Substrate, 250 mL Kit - FAQs

查看更多产品信息 SuperSignal™ ELISA Femto Substrate - FAQs (37074, 37075)

8 个常见问题解答

What type of instrument do I use to read the plates detected with the SuperSignal ELISA Femto Maximum Sensitivity Substrate?

A luminometer is used to measure relative light units.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What microplates work best with the SuperSignal ELISA Femto Maximum Sensitivity Substrate?

White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal. Here are two suggestions we offer for white plates:

-Pierce 96-Well Polystyrene Plates, White Opaque (Cat. No. 15042)
-Nunc FluoroNunc/LumiNunc 96-Well Plates (Cat. No. 437796)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What signal duration occurs with SuperSignal ELISA Femto Maximum Sensitivity Substrate?

Maximum signal is reached within two minutes and then declines steadily until it is neglible after twenty minutes.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How sensitive is SuperSignal ELISA Femto Maximum Sensitivity Substrate?

Our researchers have detected down to 168 fg of interleukin-2 in sandwich ELISA.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I dilute the SuperSignal ELISA Femto Maximum Sensitivity Substrate working reagent if my signal is too intense?

We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

At what wavelength is SuperSignal ELISA Femto Maximum Sensitivity Substrate measured?

The light emission wavelength is measured at 425 nm.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Have you tested the SuperSignal ELISA Femto Substrate with magnetic beads?

We have not tested the SuperSignal ELISA Femto Substrate with magnetic beads. However, we know that some metals can interfere with the substrate. Therefore, depending on the chemistry of the beads, they may or may not interfere with the substrate.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Is the time taken to add your SuperSignal ELISA Femto Substrate to the wells of a microplate important?

Yes. The signal from the SuperSignal ELISA Femto Substrate ramps up very quickly and optimal signal production lasts only for 5-10 min. Therefore, we recommend using a multichannel pipette to add the substrate to the wells, mix for 1 min, and then read the signal produced. Typically, reading each well for 0.1-1 sec will provide good signal with this substrate.
Note: With the SuperSignal ELISA Pico Chemiluminescent Substrate (Cat. No. 37070, 37069), the signal does not decay as quickly and researchers typically have up to 30 min to read the plate.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.