TaqMan™ GAPDH Control Reagents (human) - FAQs

查看更多产品信息 TaqMan™ GAPDH Control Reagents (human) - FAQs (402869)

3 个常见问题解答

In the TaqMan GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?

The amplicon length is 226 bases, and is based on the NCBI GenBank accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the “TaqMan Gold RT-PCR Kit” protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

Do your human and rodent GAPDH primers and TaqMan probe sequences detect genomic DNA?

It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:

- The TaqMan GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE dye, spans two exons therefore will not amplify genomic DNA.

- The Human GAPD (GAPDH) Endogenous Control (VIC/ TAMRA Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.

- The probe in the TaqMan Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC dye, does not span an intron and therefore may amplify genomic DNA.