TaqMan™ 啮齿动物 GAPDH 对照试剂
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Applied Biosystems™

TaqMan™ 啮齿动物 GAPDH 对照试剂

Applied Biosystems™ TaqMan™ 啮齿动物 GAPDH 对照试剂可提供用于在大鼠、小鼠及中国仓鼠细胞中使用啮齿动物 GAPDH 作为归一化对照的组分。•预设计的引物和了解更多信息
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货号数量
43083131,000 reactions
货号 4308313
价格(CNY)
9,861.00
Each
添加至购物车
数量:
1,000 reactions
价格(CNY)
9,861.00
Each
添加至购物车
Applied Biosystems™ TaqMan™ 啮齿动物 GAPDH 对照试剂可提供用于在大鼠、小鼠及中国仓鼠细胞中使用啮齿动物 GAPDH 作为归一化对照的组分。

•预设计的引物和 TaqMan™ 探针无需检测设计
•《快速测定开发指南》可以大大缩短优化时间
•单独的组分可为检测设置提供灵活性

包括综合指南
Applied Biosystems™ 已制定一套综合指南,确保正确使用 Applied Biosystems™ 实时荧光定量 PCR 试剂和仪器。这些指南简单易行。许多传统变量,例如氯化镁浓度和热循环方案本身已经标准化,极大地缩短了测定开发时间。

内置标准化
所有 Applied Biosystems™ 实时荧光定量 PCR 试剂盒均包含了被动内部参比,以对非 PCR 相关荧光波动进行标准化。使用被动内参进行标准化将尽可能地减少各种原因(例如移液误差和样品蒸发)导致的孔间差异。当多个探针(不同的报告基团染料)合并于单管中时,惰性参比对结果的准确性也很关键。

使用说明:
TaqMan™ 啮齿动物 GAPDH 对照试剂可与 TaqMan™ 通用 PCR 预混液或 TaqMan™ PCR 核心试剂联合用于聚合酶链反应 (PCR)。TaqMan™ 啮齿动物 GAPDH 对照试剂可与 TaqMan™ 一步法 RT-PCR 预混液试剂、TaqMan™ EZ RT-PCR 核心试剂或 TaqMan™ Gold RT-PCR 试剂联合用于一步逆转录聚合酶链反应 (RT-PCR)。

注意:
有关有限标签许可和商标信息,请参阅用户手册或产品说明书。仅供科研使用。不适用于诊断用途。
仅供科研使用。不可用于诊断程序。
规格
控制模板对照小鼠 RNA
产品规格管装
基因符号GAPDH
内部探针修改TAMRA™ 淬灭剂 (3')、VIC™ (5')
反应次数1000 次反应
产品线TaqMan
产品类型实时荧光定量 PCR 试剂
数量1,000 reactions
运输条件干冰
种属小鼠、仓鼠、大鼠
足够用于1000 次反应
靶标GAPDH
高 GC PCR 扩增效果
标签或染料VIC:
PCR 方法一步法 RT-qPCR
反应速度标准
Unit SizeEach
内容与储存
对照引物探针套件&模板

常见问题解答 (FAQ)

What type of control RNA is included in the TaqMan Rodent GAPDH kit (P/N 4308313)?

The RNA included in the TaqMan Rodent GAPDH kit is total RNA extracted from P388D1 lymphoma mouse cell line.

What is the GenBank accession number of the sequence that was used to design the primers and probe for the TaqMan Rodent GAPDH Control Reagent Kit (P/N 4308313)?

The GenBank accession number used is NM_008084. For more information on this kit, please refer to the TaqMan Rodent GAPDH kit protocol.

Since the TaqMan Rodent GAPDH Control Reagents (Cat. No. 4308313) will also amplify pseudogenes and/or genomic DNA, is there some equation that can be used to correct for genomic DNA contamination?

There is no equation for the correction of data for genomic contamination; however, a rule of thumb is that if the Ct difference between your target and the contaminant is 10 or more, you can assume it is not having an effect in your reaction. Please consult the TaqMan Rodent GAPDH Control Reagents Protocol for more information on these reagents. Enter the catalog number in the main Search field on our web site to view the product detail page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

In the TaqMan Rodent GAPDH Control Reagents kit (P/N 4308313), what rodent is the Control RNA isolated from?

The control RNA is total RNA isolated from a mouse lymphoma cell line.